Typical Testing Data/Standard Curve (for reference only)
(Mouse IL-11 PicoKine ELISA Kit standard curve )
Mouse IL-11 PicoKine ELISA Kit | Il11 elisa kit
Mouse IL-11 PicoKine ELISA Kit
Gene Names
Il11; IL-11
Reactivity
Mouse
Synonyms
IL-11 PicoKine; Mouse IL-11 PicoKine ELISA Kit; Interleukin-11; Adipogenesis inhibitory factor; AGIF; IL 11; IL-11; Il11; IL11_HUMAN; Interleukin 11; Interleukin11; Oprelvekin; interleukin 11; Il11 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse IL-11
Sequence Length
199
Assay Range
31.2pg/ml-2000pg/ml
Sensitivity
<10pg/ml
Sample
For quantitative detection of mouse IL-11 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Immunogen
E Coli, P22-L199
Assay Procedure Step 1
Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse IL-11 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each mouse IL-11 standard solution and each sample be measured in duplicate.
Intra-Assay Precision
Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|138|716|1354|146|727|1379
Standard deviation|6.07|36.52|86.66|10.51|53.8|113.1
CV(%)|4.4|5.1|6.4|7.2|7.4|8.2
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|138|716|1354|146|727|1379
Standard deviation|6.07|36.52|86.66|10.51|53.8|113.1
CV(%)|4.4|5.1|6.4|7.2|7.4|8.2
Sample Data
Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000
O.D.|0.053|0.145|0.229|0.392|0.633|1.122|1.839|2.510
O.D.|0.053|0.145|0.229|0.392|0.633|1.122|1.839|2.510
Anticoagulant
Heparin, EDTA or Citrate
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
(Mouse IL-11 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse IL-11 PicoKine ELISA Kit standard curve )
Related Product Information for Il11 elisa kit
Principle of the assay: Mouse IL-11 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-11 has been precoated onto 96-well plates. Standards(E Coli, P22-L199) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-11 amount of sample captured in plate.
Background: Interleukin 11(IL-11) is a protein that in humans is encoded by the IL11 gene. The protein encoded by this gene is a member of the gp130 family of cytokines. This gene is mapped to 19q13.42. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. What's more, IL-11 can stimulate the growth of certain lymphocytes and it has functions in many other tissues, including the brain, gut, testis and bone. In transgenic mice, overexpression of the human IL11 gene resulted in the stimulation of bone formation to increase cortical thickness and strength of long bones.
Background: Interleukin 11(IL-11) is a protein that in humans is encoded by the IL11 gene. The protein encoded by this gene is a member of the gp130 family of cytokines. This gene is mapped to 19q13.42. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. What's more, IL-11 can stimulate the growth of certain lymphocytes and it has functions in many other tissues, including the brain, gut, testis and bone. In transgenic mice, overexpression of the human IL11 gene resulted in the stimulation of bone formation to increase cortical thickness and strength of long bones.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,522 Da
NCBI Official Full Name
interleukin-11 isoform 1
NCBI Official Synonym Full Names
interleukin 11
NCBI Official Symbol
Il11
NCBI Official Synonym Symbols
IL-11
NCBI Protein Information
interleukin-11
UniProt Protein Name
Interleukin-11
Protein Family
UniProt Gene Name
Il11
UniProt Synonym Gene Names
IL-11
UniProt Entry Name
IL11_MOUSE
Uniprot Description
IL11: Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Belongs to the IL-6 superfamily.
Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Cell cycle regulation; Cytokine
Cellular Component: cytoplasm; extracellular region; extracellular space
Molecular Function: cytokine activity; growth factor activity; interleukin-11 receptor binding
Biological Process: negative regulation of hormone secretion; positive regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription from RNA polymerase II promoter
Research Articles on Il11
Similar Products
Product Notes
The Mouse Il11 il11 (Catalog #AAA177597) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177597 ELISA Kit recognizes Mouse Il11. It is sometimes possible for the material contained within the vial of "IL-11 PicoKine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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