Rat DLL4 ELISA Kit | DLL4 elisa kit

Rat DLL4 PicoKine ELISA Kit

Cat. Num. AAA178756

• Reactivity: Rat
• Synonyms: DLL4; Rat DLL4 PicoKine ELISA Kit; Delta 4; Delta4; Delta ligand 4; Delta like 4; DLL 4; hdelta2; Homeobox protein DLL-4; Notch ligand delta 2; Notch ligand DLL4; XDLL-4; Q9NR61; delta-like 4 (Drosophila); DLL4 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat DLL4
• Sequence Length: 686

• Assay Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Anticoagulant: heparin or EDTA
• TMB incubation time: 25-30
• Immunogen: NSO, S28-E519
• Sample Data: Concentration(pg/ml): |0|15.6|31.2|62.5|125|250|500|1000; O.D.: |0.008|0.108|0.203|0.377|0.672|1.188|1.840|2.443
• Intra/Inter Assay Precision: Sample: |1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml): |48.9|157|524|50.2|189|601; Standard Deviation: |2.30|8.01|30.39|2.66|11.15|40.27; CV(%): |4.7|5.1|5.8|5.3|5.9|6.7
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml rat DLL4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. It is recommended that each rat DLL4 standard solution and each sample be measured in duplicate.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Rat DLL4 PicoKine ELISA Kit standard curve )

Related Product Information for DLL4 elisa kit

For quantitative detection of rat DLL4 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Delta like ligand 4 is a protein that in humans is encoded by the DLL4 gene. It is mapped to 15q15.1. This gene is a homolog of the Drosophila delta gene. DLL4 is a transmembrane ligand for Notch receptors that shows restricted expression to endothelial cells (ECs), in particular to arteries and capillaries, and is involved in vascular development, and it also appeared to be a major trigger of Notch receptor activities previously implicated in arterial and vascular development. DLL4 acts as a negative regulator of tumor angiogenesis, its blockade results in the striking uncoupling of tumor growth from vessel density, presenting a novel therapeutic approach even for tumors resistant to anti-VEGF therapies. In addition to it, this gene also plays an important role in promoting Th17 effector activity during mycobacterial challenge.
Principle: Rat DLL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for DLL4 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: S28-E519) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DLL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat DLL4 amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GI #: 564342382
• NCBI GeneID: 311332
• NCBI Accession #: XP_006234827.1
• NCBI GenBank Nucleotide #: XM_006234765.2
• UniProt Accession #: D3ZHH1
• Molecular Weight: 74,961 Da
• NCBI Official Full Name: delta-like protein 4 isoform X1
• NCBI Official Synonym Full Names: delta like canonical Notch ligand 4
• NCBI Official Symbol: Dll4
• NCBI Protein Information: delta-like protein 4
• UniProt Protein Name: Delta-like protein 4
• Protein Family: Delta-like protein
• UniProt Gene Name: Dll4
• UniProt Synonym Gene Names: Delta4Curated

Uniprot Description

Involved in the Notch signaling pathway as Notch ligand. Activates NOTCH1 and NOTCH4 (PubMed:25700513). Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types. During spinal cord neurogenesis, inhibits V2a interneuron fate ().