Rat ALCAM ELISA Kit | ALCAM elisa kit

Rat ALCAM PicoKine ELISA Kit

Cat. Num. AAA178758

• Reactivity: Rat
• Synonyms: ALCAM; Rat ALCAM PicoKine ELISA Kit; ALCAM protein; CD166 antigen; Protein MEMD; Q13740; activated leukocyte cell adhesion molecule; ALCAM elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat ALCAM
• Sequence Length: 583

• Assay Range: 78pg/ml-5000pg/ml
• Sensitivity: <10pg/ml
• Anticoagulant: heparin or EDTA
• TMB incubation time: 25-30
• Immunogen: NSO, W28-K527
• Sample Data: Concentration(pg/ml): |0|78|156|312|625|1250|2500|5000; O.D.: |0.004|0.064|0.118|0.215|0.450|0.798|1.578|2.317
• Intra/Inter Assay Precision: Sample: |1|2|3|1|2|3; n: |16|16|16|24|24|24; Mean(pg/ml): |189|722|2741|201|830|2905; Standard Deviation: |9.26|37.54|158.98|11.86|50.63|203.35; CV(%): |4.9|5.2|5.8|5.9|6.1|7.0
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml, 78pg/mll rat ALCAM standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. It is recommended that each rat ALCAM standard solution and each sample be measured in duplicate.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Rat ALCAM PicoKine ELISA Kit standard curve )

Related Product Information for ALCAM elisa kit

For quantitative detection of rat ALCAM in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The human gene was mapped to chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. This gene is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.
Principle: Rat ALCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for ALCAM has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: W28-K527) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ALCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat ALCAM amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GI #: 13929058
• NCBI GeneID: 79559
• NCBI Accession #: NP_113941.1
• NCBI GenBank Nucleotide #: NM_031753.1
• UniProt Accession #: O35112; O55172
• Molecular Weight: 65,022 Da
• NCBI Official Full Name: CD166 antigen
• NCBI Official Synonym Full Names: activated leukocyte cell adhesion molecule
• NCBI Official Symbol: Alcam
• NCBI Protein Information: CD166 antigen
• UniProt Protein Name: CD166 antigen
• Protein Family: CD166 antigen
• UniProt Gene Name: Alcam

Uniprot Description

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (). Mediates homotypic interactions with cells that express ALCAM. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM (). Mediates outgrowth and pathfinding for retinal ganglion cell axons ().