Mouse DKK4 ELISA Kit | Dkk4 elisa kit

Mouse DKK4 PicoKine ELISA Kit

Cat. Num. AAA178764

• Gene Names: Dkk4; Dkk-4
• Reactivity: Mouse
• Synonyms: DKK4; Mouse DKK4 PicoKine ELISA Kit; Dickkopf 4; Dickkopf gene 4; Dickkopf homolog 4; Dickkopf related protein 4; Dickkopf4; DKK 4; hDkk4; MGC129562; MGC129563; MGC25705; RGD1563172; Q9UBT3; dickkopf WNT signaling pathway inhibitor 4; Dkk4 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse DKK4
• Sequence Length: 221

• Assay Range: 78pg/ml-5000pg/ml
• Sensitivity: <10pg/ml
• Anticoagulant: heparin or EDTA
• TMB incubation time: 25-30
• Immunogen: NSO, L19-I221
• Sample Data: Concentration(pg/ml): |0|78|156|312|625|1250|2500|5000; O.D.: |0.011|0.042|0.073|0.129|0.247|0.534|1.147|2.304
• Intra/Inter Assay Precision: Sample: |1|2|3|1|2|3; n: |16|16|16|24|24|24; Mean(pg/ml): |103|495|1943|81|513|2209; Standard Deviation: |8.13|23.76|102.98|5.59|27.18|92.78; CV(%): |7.9|4.8|5.3|6.9|5.3|4.2
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml, 78pg/mll mouse DKK4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. It is recommended that each mouse DKK4 standard solution and each sample be measured in duplicate.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse DKK4 PicoKine ELISA Kit standard curve )

Related Product Information for Dkk4 elisa kit

For quantitative detection of mouse DKK4 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: DKK4 is a membersof the Dickkopf family which are negative regulators of the growth-promoting Wnt / beta-catenin signaling pathway. By FISH, the DKK4 gene was mapped to chromosome 8p11.2-p11.1. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. The expression of DKK4 was significantly reduced in hepatocellular carcinomas (HCCs), particularly in HCCs that showed cytoplasmic beta- catenin accumulation. DKK4 expression was also reduced in HCC cell lines. Ectopic expression of DKK4 in 2 HCC cell lines reduced beta-catenin and cyclin D1 protein levels and attenuated beta-catenin activity in reporter gene assays.
Principle: Mouse DKK4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Rat specific for DKK4 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: L19-I221) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse DKK4 amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GI #: 21704186
• NCBI GeneID: 234130
• NCBI Accession #: NP_663567.1
• NCBI GenBank Nucleotide #: NM_145592.2
• UniProt Accession #: Q8VEJ3
• Molecular Weight: 24,261 Da
• NCBI Official Full Name: dickkopf-related protein 4
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 4
• NCBI Official Symbol: Dkk4
• NCBI Official Synonym Symbols: Dkk-4
• NCBI Protein Information: dickkopf-related protein 4
• UniProt Protein Name: Dickkopf-related protein 4
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: Dkk4
• UniProt Synonym Gene Names: Dickkopf-4; Dkk-4

Uniprot Description

DKK4: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8|8 A2

Biological Process: Wnt receptor signaling pathway