Rat DLL4 ELISA Kit | DLL4 elisa kit

Rat DLL4 ELISA Kit

Cat. Num. AAA8248477

• Reactivity: Rat
• Synonyms: DLL4; Rat DLL4 ELISA Kit; Delta-like protein 4; Drosophila Delta homolog 4; Delta4; DLL4 elisa kit

• Reactivity: Rat
• Specificity: The Rat DLL4 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat DLL4 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 686

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DLL4 elisa kit

Principle of the Assay: The Rat DLL4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat DLL4 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Rat DLL4 coated on a 96-well plate. Standards and samples are pipetted into the wells and DLL4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat DLL4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DLL4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Delta like ligand 4 is a protein that in humans is encoded by the DLL4 gene. It is mapped to 15q15.1. This gene is a homolog of the Drosophila delta gene. DLL4 is a transmembrane ligand for Notch receptors that shows restricted expression to endothelial cells (ECs), in particular to arteries and capillaries, and is involved in vascular development, and it also appeared to be a major trigger of Notch receptor activities previously implicated in arterial and vascular development. DLL4 acts as a negative regulator of tumor angiogenesis, its blockade results in the striking uncoupling of tumor growth from vessel density, presenting a novel therapeutic approach even for tumors resistant to anti-VEGF therapies. In addition to it, this gene also plays an important role in promoting Th17 effector activity during mycobacterial challenge.

NCBI and Uniprot Product Information

• NCBI GI #: 564342382
• NCBI GeneID: 311332
• NCBI Accession #: XP_006234827.1
• NCBI GenBank Nucleotide #: XM_006234765.2
• UniProt Accession #: D3ZHH1
• Molecular Weight: 74,961 Da
• NCBI Official Full Name: delta-like protein 4 isoform X1
• NCBI Official Synonym Full Names: delta like canonical Notch ligand 4
• NCBI Official Symbol: Dll4
• NCBI Protein Information: delta-like protein 4
• UniProt Protein Name: Delta-like protein 4
• Protein Family: Delta-like protein
• UniProt Gene Name: Dll4
• UniProt Synonym Gene Names: Delta4Curated
• UniProt Entry Name: DLL4_RAT

Uniprot Description

Involved in the Notch signaling pathway as Notch ligand. Activates NOTCH1 and NOTCH4 (PubMed:25700513). Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types. During spinal cord neurogenesis, inhibits V2a interneuron fate ().