Mouse DAN/NBL1 PicoKine ELISA Kit | Nbl1 elisa kit

Mouse DAN/NBL1 PicoKine ELISA Kit

Cat. Num. AAA177614

• Gene Names: Nbl1; DAN; NO3; Dana; D4H1S1733E
• Reactivity: Mouse
• Synonyms: DAN/NBL1 PicoKine; Mouse DAN/NBL1 PicoKine ELISA Kit; Neuroblastoma suppressor of tumorigenicity 1; D1S1733E; DAN; DAN domain family member 1; DAND 1; DAND1; Differential screening selected gene aberrant in neuroblastoma; MGC8972; NB; NBL 1; Neuroblastoma candidate region suppression of tumorigenicity 1; Neuroblastoma suppression of tumorigenicity 1; NO 3; NO3; Zinc finger protein DAN ; neuroblastoma 1; DAN family BMP antagonist; Nbl1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse DAN
• Sequence Length: 178

• Assay Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of Mouse DAN in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: NSO, A17-D178
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse DAN standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse DAN standard solution and each sample is measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|675|1810|2944|792|1948|3107; Standard deviation|37.12|112.22|197.24|50.68|146.10|242.34; CV(%)|5.5|6.2|6.7|6.4|7.5|7.8
• Sample Data: Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000; O.D.|0.023|0.080|0.152|0.267|0.477|0.864|1.579|2.313
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse DAN/NBL1 PicoKine ELISA Kit standard curve )

Related Product Information for Nbl1 elisa kit

Principle of the assay: Mouse DAN ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for DAN has been precoated onto 96-well plates. Standards (NSO, A17-D178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DAN is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse DAN amount of sample captured in plate.

Background: Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

NCBI and Uniprot Product Information

• NCBI GI #: 148234601
• NCBI GeneID: 17965
• NCBI Accession #: NP_032701.2
• NCBI GenBank Nucleotide #: NM_008675.2
• UniProt Accession #: Q61477; Q499K5
• Molecular Weight: 19,107 Da
• NCBI Official Full Name: neuroblastoma suppressor of tumorigenicity 1
• NCBI Official Synonym Full Names: neuroblastoma, suppression of tumorigenicity 1
• NCBI Official Symbol: Nbl1
• NCBI Official Synonym Symbols: DAN; NO3; Dana; D4H1S1733E
• NCBI Protein Information: neuroblastoma suppressor of tumorigenicity 1
• UniProt Protein Name: Neuroblastoma suppressor of tumorigenicity 1
• Protein Family: N-terminal-borealin-like protein
• UniProt Gene Name: Nbl1
• UniProt Synonym Gene Names: Dan; Dana
• UniProt Entry Name: NBL1_MOUSE

Uniprot Description

NBL1: Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process. Belongs to the DAN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Secreted; Secreted, signal peptide

Cellular Component: extracellular region

Molecular Function: morphogen activity; protein binding; protein homodimerization activity

Biological Process: determination of dorsal identity; negative regulation of BMP signaling pathway; nervous system development; neurite morphogenesis; positive regulation of neuron differentiation