Mouse LIF ELISA Kit | LIF elisa kit

Mouse LIF PicoKine ELISA Kit

Cat. Num. AAA178717

• Reactivity: Mouse
• Synonyms: LIF; Mouse LIF PicoKine ELISA Kit; CDF; Cholinergic differentiation factor; D factor; DIA; Differentiation inhibitory activity; Differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; Hepatocyte stimulating factor III; HILDA; Leukemia inhibitory factor; Melanoma derived LPL inhibitor; Melanoma-derived LPL inhibitor; MLPL I; P15018; LIF elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse LIF
• Sequence Length: 158

• Assay Range: 7.8pg/ml-500pg/ml
• Sensitivity: <10pg/ml
• Anticoagulant: heparin or EDTA
• TMB incubation time: 25-30
• Immunogen: E Coli, S24-F203
• Sample Data: Concentration(pg/ml): |0|7.8|15.6|31.3|62.5|125|250|500; O.D.: |0.008|0.062|0.141|0.295|0.574|1.100|1.981|2.761
• Intra/Inter Assay Precision: Sample: |1|2|3|1|2|3; n: |16|16|16|24|24|24; Mean(pg/ml): |12.2|50|197|9.8|44.1|201; Standard Deviation: |0.75|2.15|10.83|0.71|2.60|12.84; CV(%): |6.2|4.3|5.5|7.3|5.9|6.4
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml mouse LIF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. We recommend that each mouse LIF standard solution and each sample is measured in duplicate.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse LIF PicoKine ELISA Kit standard curve )

Related Product Information for LIF elisa kit

For quantitative detection of mouse LIF in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The LIF was mapped gene to 22q11-q12.2 by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of 2 normal males and some individuals with chromosomal rearrangements. The gene maps between the Philadelphia translocation BCR1 and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation.
Principle: Mouse LIF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Rat specific for LIF has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, Immunogen sequence: S24-F203) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LIF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse LIF amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GI #: 88014736
• NCBI GeneID: 16878
• NCBI Accession #: NP_001034626.1
• NCBI GenBank Nucleotide #: NM_001039537.2
• UniProt Accession #: P09056
• Molecular Weight: 22,287 Da
• NCBI Official Full Name: leukemia inhibitory factor isoform b
• NCBI Official Synonym Full Names: leukemia inhibitory factor
• NCBI Official Symbol: Lif
• NCBI Protein Information: leukemia inhibitory factor
• UniProt Protein Name: Leukemia inhibitory factor
• Protein Family: Leukemia inhibitory factor
• UniProt Gene Name: Lif
• UniProt Synonym Gene Names: LIF; D factor

Uniprot Description

LIF: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. Belongs to the LIF/OSM family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11 A1|11 2.94 cM

Cellular Component: cytoplasm; extracellular space

Molecular Function: cytokine activity; growth factor activity; leukemia inhibitory factor receptor binding; receptor binding

Biological Process: alveolus development; astrocyte differentiation; blood vessel remodeling; decidualization; embryo implantation; leukemia inhibitory factor signaling pathway; lung development; maternal process involved in pregnancy; muscle morphogenesis; negative regulation of angiogenesis; negative regulation of cell proliferation; negative regulation of hormone secretion; negative regulation of meiosis; neuron development; organ regeneration; positive regulation of adrenocorticotropic hormone secretion; positive regulation of astrocyte differentiation; positive regulation of cell proliferation; positive regulation of macrophage differentiation; positive regulation of MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-serine phosphorylation of STAT protein; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of cell differentiation; retina development in camera-type eye; stem cell differentiation; stem cell maintenance; tyrosine phosphorylation of Stat3 protein