Rat RANTES CLIA Kit | RANTES clia kit

RANTES (Rat) LumiAb ELISA Kit

Cat. Num. AAA9510867

• Gene Names: Ccl5; Scya5; Rantes
• Reactivity: Rat
• Synonyms: RANTES; RANTES (Rat) LumiAb ELISA Kit; Rat RANTES; C-C motif chemLumine 5; SIS-delta; Small-inducible cytLumine A5; T-cell-specific protein RANTES; Scya5; RANTES clia kit

• Reactivity: Rat
• Specificity: The LumiAb Rat MCP-1 ELISA is capable of recognizing both recombinant and naturally produced Rat MCP-1 proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.
• Sequence Length: 92

• Assay Type: Quantitative Sandwich
• Sensitivity: 15 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RANTES clia kit

Principle of the Assay: The LumiAb Rat MCP-1 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Rat MCP-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Rat MCP-1 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Rat MCP-1 or Monocyte Chemotactic Protein 1, also known as C-C Motif Chemokine 2, is a 148 amino acid cytokine protein encoded by the Ccl2 gene located at locus 10q26 on chromosome 10. After initial synthesis, the 23 residue signal sequence is cleaved from the N-terminal region, allowing for proper folding and maturation of the MCP-1 peptide. MCP-1, a monomeric or homodimeric intercrine beta (chemokine C-C) family member is known to be a chemotactic factor that attracts monocytes but not neutrophils. Structurally, it consists of 2 disulfide linkages and a possible glycosylation site at residue 126.

Product Categories/Family for RANTES clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 1711393
• NCBI GeneID: 81780
• UniProt Accession #: P50231
• NCBI Official Full Name: C-C motif chemokine 5
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 5
• NCBI Official Symbol: Ccl5
• NCBI Official Synonym Symbols: Scya5; Rantes
• NCBI Protein Information: C-C motif chemokine 5
• UniProt Protein Name: C-C motif chemokine 5
• UniProt Gene Name: Ccl5
• UniProt Synonym Gene Names: Scya5
• UniProt Entry Name: CCL5_RAT

NCBI Description

may play a role in cellular response to viral infection in testicular somatic cells [RGD, Feb 2006]

Uniprot Description

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.