Human MCP-1 CLIA Kit | MCP-1 clia kit

MCP-1 (Human) LumiAb ELISA Kit

Cat. Num. AAA9510802

• Gene Names: CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
• Reactivity: Human
• Synonyms: MCP-1; MCP-1 (Human) LumiAb ELISA Kit; Human MCP-1; C-C motif chemLumine 2; HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Small-inducible cytLumine A2; SCYA2; MCP-1 clia kit

• Reactivity: Human
• Specificity: The LumiAb Human Leptin ELISA is capable of recognizing both recombinant and naturally produced Human Leptin proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 5% cross reactivity.
• Sequence Length: 99

• Assay Type: Quantitative Sandwich
• Sensitivity: 15 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MCP-1 clia kit

Principle of the Assay: The LumiAb Human Leptin Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Leptin concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Leptin while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human Leptin, also known as Obesity Factor, is a 167 amino acid cytokine protein encoded by the LEP gene located at locus 7q31.3 on chromosome 7. Leptin may function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity, a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the results of excessive accumulation of body fat. Leptin also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in the gene and/or its regulatory regions may cause severe obesity and morbid obesity with hypogonadism as well as development of type 2 diabetes mellitus.

Product Categories/Family for MCP-1 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 545465
• NCBI GeneID: 6347
• NCBI Official Full Name: MCP-1
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 2
• NCBI Official Symbol: CCL2
• NCBI Official Synonym Symbols: HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
• NCBI Protein Information: C-C motif chemokine 2
• Protein Family: Major capsid protein

NCBI Description

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]