Mouse KC CLIA Kit | KC clia kit

KC (Murine) LumiAb ELISA Kit

Cat. Num. AAA9510844

• Gene Names: Cxcl1; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
• Reactivity: Mouse
• Synonyms: KC; KC (Murine) LumiAb ELISA Kit; Murine KC; Growth-regulated alpha protein; C-X-C motif chemLumine 1; Platelet-derived growth factor-inducible protein KC; Secretory protein N51; KC clia kit

• Reactivity: Mouse
• Specificity: The LumiAb Murine JE/MCP-1 ELISA is capable of recognizing both recombinant and naturally produced Murine JE/MCP-1 proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.
• Sequence Length: 96

• Assay Type: Quantitative Sandwich
• Sensitivity: 15 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KC clia kit

Principle of the Assay: The LumiAb Murine JE/MCP-1 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine JE/MCP-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Murine JE/MCP-1 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Murine JE/MCP-1 or Monocyte Chemotactic Protein 1, mainly known as C-C Motif Chemokine 2, is a 148 amino acid cytokine protein expressed from chromosome 11. After initial synthesis and translocation, the 23 residue signal sequence is cleaved, allowing the 125 residue JE/MCP-1 peptide to properly fold and mature. Belonging to the intercrine beta (chemokine C-C) family, the monomeric and homodimeric JE/MCP-1 proteins function as chemotactic factors that attract monocytes, but not neutrophils. Furthermore, they are typically induced by platelet-derived growth factors (PDGF).

Product Categories/Family for KC clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 6680109
• NCBI GeneID: 14825
• NCBI Accession #: NP_032202.1
• NCBI GenBank Nucleotide #: NM_008176.3
• UniProt Accession #: P12850
• NCBI Official Full Name: growth-regulated alpha protein
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 1
• NCBI Official Symbol: Cxcl1
• NCBI Official Synonym Symbols: KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
• NCBI Protein Information: growth-regulated alpha protein
• UniProt Protein Name: Growth-regulated alpha protein
• Protein Family: KCNQ1 downstream neighbor protein
• UniProt Gene Name: Cxcl1
• UniProt Synonym Gene Names: Gro; Gro1; Mgsa; Scyb1; HSF
• UniProt Entry Name: GROA_MOUSE

NCBI Description

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung. [provided by RefSeq, Apr 2013]

Uniprot Description

CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding; cytokine activity

Biological Process: G-protein coupled receptor protein signaling pathway; neutrophil chemotaxis; elevation of cytosolic calcium ion concentration; positive regulation of superoxide release; response to molecule of bacterial origin; positive regulation of potassium ion transport; immune response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; inflammatory response; acute inflammatory response; regulation of cell proliferation