Human IL-17F CLIA Kit | IL-17F clia kit

IL-17F (Human) LumiAb ELISA Kit

Cat. Num. AAA9510783

• Gene Names: IL17F; ML1; ML-1; CANDF6; IL-17F
• Reactivity: Human
• Synonyms: IL-17F; IL-17F (Human) LumiAb ELISA Kit; Human IL-17F; Interleukin-17F; CytLumine ML-1; Interleukin-24; IL-17F clia kit

• Reactivity: Human
• Specificity: The LumiAb Human IL-17E ELISA is capable of recognizing both recombinant and naturally produced Human IL-17E proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.
• Sequence Length: 163

• Assay Type: Quantitative Sandwich
• Sensitivity: 4 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-17F clia kit

Principle of the Assay: The LumiAb Human IL-17E Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human IL-17E concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human IL-17E while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human IL-17E or IL-25 is a cytokine that shares sequence similarity with IL-17, induces activation of NF-kappaB while also stimulating the production of proinflammatory chemokine IL-8, and favors Th2-type immune responses. The cytokine itself is expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland and trachea. Encoded by the IL25 gene located at locus 14q11.2 on chromosome 14, IL-17E and IL-17B are both ligands for the cytokine receptor, IL17BR. After initial synthesis of the protein, the IL-17E preprotein undergoes proteolytic processing and cleavage, forming the 32 residue signal sequence along with the actual 145 IL-17E peptide that is then allowed to fold and mature.

Product Categories/Family for IL-17F clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 16418375
• NCBI GeneID: 112744
• NCBI Accession #: NP_443104.1
• NCBI GenBank Nucleotide #: NM_052872.3
• UniProt Accession #: Q96PD4
• NCBI Official Full Name: interleukin-17F
• NCBI Official Synonym Full Names: interleukin 17F
• NCBI Official Symbol: IL17F
• NCBI Official Synonym Symbols: ML1; ML-1; CANDF6; IL-17F
• NCBI Protein Information: interleukin-17F
• UniProt Protein Name: Interleukin-17F
• UniProt Gene Name: IL17F
• UniProt Synonym Gene Names: IL-17F
• UniProt Entry Name: IL17F_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. [provided by RefSeq, Jul 2008]

Uniprot Description

IL17F: Stimulates the production of other cytokines such as IL- 6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6). CANDF6 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. Belongs to the IL-17 family.

Protein type: Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: extracellular space; extracellular region

Molecular Function: protein homodimerization activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine binding; cytokine activity

Biological Process: proteoglycan metabolic process; negative regulation of angiogenesis; regulation of transforming growth factor beta receptor signaling pathway; regulation of interleukin-8 biosynthetic process; lymphotoxin A biosynthetic process; cartilage development; cytokine biosynthetic process; regulation of interleukin-2 biosynthetic process; regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; regulation of interleukin-6 biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; inflammatory response

Disease: Candidiasis, Familial, 6