Human MIP-1alpha CLIA Kit | MIP-1alpha clia kit

MIP-1alpha (Human) LumiAb ELISA Kit

Cat. Num. AAA9510806

• Gene Names: CCL3; MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
• Reactivity: Human
• Synonyms: MIP-1alpha; MIP-1alpha (Human) LumiAb ELISA Kit; Human MIP-1alpha; C-C motif chemLumine 3; G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; PAT 464.1; SIS-beta; Small-inducible cytLumine A3; Tonsillar lymphocyte LD78 alpha protein; SCYA3; G0S19-1; MIP-1alpha clia kit

• Reactivity: Human
• Specificity: The LumiAb Human MIG ELISA is capable of recognizing both recombinant and naturally produced Human MIG proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.
• Sequence Length: 70

• Assay Type: Quantitative Sandwich
• Sensitivity: 4 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MIP-1alpha clia kit

Principle of the Assay: The LumiAb Human MIG Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human MIG concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human MIG while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human MIG or Monokine Induced by IFN-gamma, also known as C-X-C Motif Chemokine 9, is a 125 amino acid chemokine protein that affects the growth, movement or activation state of cells that participate in immune and inflammatory response. MIG is expressed from the CXCL9 gene located at locus 4q21 on chromosome 4. Via its binding with CXCR3, MIG is chemotactic for activated T-cells and is thought to be involved in T-cell trafficking.

Product Categories/Family for MIP-1alpha clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 2905628
• NCBI GeneID: 6348
• UniProt Accession #: P10147
• NCBI Official Full Name: macrophage inflammatory protein 1 alpha, partial
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 3
• NCBI Official Symbol: CCL3
• NCBI Official Synonym Symbols: MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
• NCBI Protein Information: C-C motif chemokine 3
• UniProt Protein Name: C-C motif chemokine 3
• UniProt Gene Name: CCL3
• UniProt Synonym Gene Names: G0S19-1; MIP1A; SCYA3; MIP-1-alpha
• UniProt Entry Name: CCL3_HUMAN

NCBI Description

This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]

Uniprot Description

CCL3: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; cytoplasm; extracellular region; intracellular; cytosol

Molecular Function: identical protein binding; CCR1 chemokine receptor binding; protein binding; chemokine activity; calcium-dependent protein kinase C activity; kinase activity; CCR5 chemokine receptor binding; protein kinase activity; chemoattractant activity; phospholipase activator activity

Biological Process: positive regulation of catalytic activity; negative regulation of osteoclast differentiation; exocytosis; behavior; response to toxin; positive regulation of calcium-mediated signaling; positive regulation of interleukin-1 beta secretion; chemotaxis; protein amino acid phosphorylation; regulation of cell shape; monocyte chemotaxis; negative regulation of bone mineralization; cell-cell signaling; positive chemotaxis; positive regulation of neuron apoptosis; calcium ion transport; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; calcium-mediated signaling; MAPKKK cascade; cytoskeleton organization and biogenesis; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; positive regulation of tumor necrosis factor production; macrophage chemotaxis; cellular calcium ion homeostasis; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; cell activation; eosinophil chemotaxis; eosinophil degranulation; regulation of sensory perception of pain; positive regulation of calcium ion transport; astrocyte cell migration; positive regulation of inflammatory response; positive regulation of cell migration

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To