Mouse IL-21 CLIA Kit | IL-21 clia kit

IL-21 (Murine) LumiAb ELISA Kit

Cat. Num. AAA9510839

• Gene Names: Il21; IL-21
• Reactivity: Mouse
• Synonyms: IL-21; IL-21 (Murine) LumiAb ELISA Kit; Murine IL-21; Interleukin-21; IL-21 clia kit

• Reactivity: Mouse
• Specificity: The LumiAb Murine IL-1alpha ELISA is capable of recognizing both recombinant and naturally produced Murine IL-1alpha proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.
• Sequence Length: 146

• Assay Type: Quantitative Sandwich
• Sensitivity: 15 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-21 clia kit

Principle of the Assay: The LumiAb Murine IL-1beta Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine IL-1beta concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Murine IL-1beta while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Murine IL-1beta, also known as Interleukin-1beta, is a 269 amino acid cytokine protein expressed from chromosome 2. Produced by activated macrophages, IL-1beta, a monomeric member of the IL-1 family, stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Moreover, the lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Product Categories/Family for IL-21 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 11140821
• NCBI GeneID: 60505
• NCBI Accession #: NP_068554.1
• NCBI GenBank Nucleotide #: NM_021782.3
• UniProt Accession #: Q9ES17
• NCBI Official Full Name: interleukin-21 isoform 2
• NCBI Official Synonym Full Names: interleukin 21
• NCBI Official Symbol: Il21
• NCBI Official Synonym Symbols: IL-21
• NCBI Protein Information: interleukin-21
• UniProt Protein Name: Interleukin-21
• UniProt Gene Name: Il21
• UniProt Synonym Gene Names: IL-21
• UniProt Entry Name: IL21_MOUSE

Uniprot Description

IL21: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cytokine; Secreted; Cell development/differentiation; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-2 receptor binding; cytokine activity

Biological Process: positive regulation of interleukin-17 production; positive regulation of natural killer cell cytokine production; cell maturation; B cell apoptosis; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of B cell differentiation; positive regulation of T cell proliferation; immune response; positive regulation of interferon-gamma biosynthetic process