Chymotrypsin C Active Protein | CTRC active protein

Chymotrypsin C Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120824

• Gene Names: CTRC; CLCR; ELA4
• Purity: >97% as determined by SDS-PAGE
• Synonyms: Chymotrypsin C; Chymotrypsin C Protein; Human; Recombinant (His Tag); Human CTRC/chymotrypsin C Protein (His Tag); CLCR Protein; ELA4 Protein; chymotrypsin C (caldecrin); CTRC active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu268

• Species: Human
• Activity: Measured by its ability to cleave the fluorogenic peptide substrate, SUC-Ala-Ala-Pro-Phe-AMC. The specific activity is >300 pmol/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Cys 17
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CTRC (Q99895)(Met 1-Leu 268) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CTRC active protein

Background: Chymotrypsin C (abbreviated for CTRC), also known as caldecrin or elastase4, is a digestive enzyme of the peptidase S1 family. This enzyme is synthesized as an inactivate chymotrypsinogen. On cleavage by trypsin into two parts that activate each other by removing two small peptides in a trans-proteolysis, chymotrypsin C produced. N-linked glycosylation of human CTRC is required for efficient folding and secretion, however, the N-linked glycan is unimportant for enzyme activity or inhibitor binding. It has been proposed that CTRC is a key regulator of digestive zymogen activation and a physiological co-activator of digestive carboxypeptidases proCPA1 and proCPA2. Mutations that abolish activity or secretion of CTRC increase the risk for chronic pancreatitis. It's speculated that CTRC might regulate pancreatic cancer cell migration in relation to cytokeratin 18 expression. The pancreatic cancer cell migration ability was downregulated in pancreatic cancer Aspc-1 cells that overexpressed CTRC, whereas the cell migration ability was upregulated in Aspc-1 cells in which CTRC was suppressed.

Product Categories/Family for CTRC active protein

Serine Proteases and Regulators

References

Lacruz RS, et al. (2011) Chymotrypsin C (caldecrin) is associated with enamel development. J Dent Res. 90 (10): 1228-33.Zhou J, et al. (2011) Chymotrypsin C mutations in chronic pancreatitis. J Gastroenterol Hepatol. 26 (8): 1238-46.Wang H, et al. (2011) Effect of chymotrypsin C and related proteins on pancreatic cancer cell migration. Acta Biochim Biophys Sin (Shanghai). 43 (5): 362-71.

NCBI and Uniprot Product Information

• NCBI GI #: 62526043
• NCBI GeneID: 11330
• NCBI Accession #: NP_009203.2
• NCBI GenBank Nucleotide #: NM_007272.2
• UniProt Accession #: Q99895; O00765; Q9NUH5; A8K082
• Molecular Weight: 25,000
• NCBI Official Full Name: chymotrypsin-C preproprotein
• NCBI Official Synonym Full Names: chymotrypsin C (caldecrin)
• NCBI Official Symbol: CTRC
• NCBI Official Synonym Symbols: CLCR; ELA4
• NCBI Protein Information: chymotrypsin-C; caldecrin; elastase 4; elastase IV; serum calcium decreasing factor
• UniProt Protein Name: Chymotrypsin-C
• Protein Family: Chymotrypsin
• UniProt Gene Name: CTRC
• UniProt Synonym Gene Names: CLCR
• UniProt Entry Name: CTRC_HUMAN

NCBI Description

This gene encodes a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CTRC: Has chymotrypsin-type protease activity and hypocalcemic activity. Genetic variations in CTRC can predispose to chronic pancreatitis by diminishing its protective trypsin-degrading activity. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Protease; EC 3.4.21.2

Chromosomal Location of Human Ortholog: 1p36.21

Molecular Function: peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis

Disease: Pancreatitis, Hereditary