Rat MCP-1 CLIA Kit | MCP-1 clia kit

MCP-1 (Rat) LumiAb ELISA Kit

Cat. Num. AAA9510865

• Gene Names: Ccl2; MCP-1; Scya2; Sigje
• Reactivity: Rat
• Synonyms: MCP-1; MCP-1 (Rat) LumiAb ELISA Kit; Rat MCP-1; C-C motif chemLumine 2; Immediate-early serum-responsive protein JE; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; Small-inducible cytLumine A2; MCP-1 clia kit

• Reactivity: Rat
• Specificity: The LumiAb Rat IL-1beta ELISA is capable of recognizing both recombinant and naturally produced Rat IL-1beta proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 5% cross reactivity.
• Sequence Length: 148

• Assay Type: Quantitative Sandwich
• Sensitivity: 11 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MCP-1 clia kit

Principle of the Assay: The LumiAb Rat IL-1beta Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Rat IL-1beta concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Rat IL-1beta while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Rat IL-1beta, also known as Interleukin-1beta, is a 268 amino acid cytokine protein encoded by the Il1b gene located at locus 3q36 on chromosome 3. After initial synthesis, it undergoes proteolytic processing to remove the 116 residue propeptide sequence from the 152 residue IL-1alpha peptide, thus allowing for proper folding and maturation. Produced by activated macrophages, IL-1beta stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1beta proteins, monomeric members of the IL-1 family, are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1beta is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Product Categories/Family for MCP-1 clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 13928714
• NCBI GeneID: 24770
• NCBI Accession #: NP_113718.1
• NCBI GenBank Nucleotide #: NM_031530.1
• UniProt Accession #: P14844
• NCBI Official Full Name: C-C motif chemokine 2
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 2
• NCBI Official Symbol: Ccl2
• NCBI Official Synonym Symbols: MCP-1; Scya2; Sigje
• NCBI Protein Information: C-C motif chemokine 2
• UniProt Protein Name: C-C motif chemokine 2
• Protein Family: Major capsid protein
• UniProt Gene Name: Ccl2
• UniProt Synonym Gene Names: Je; Mcp1; Scya2; MCP-1
• UniProt Entry Name: CCL2_RAT

NCBI Description

a monocyte chemoattractant protein [RGD, Feb 2006]

Uniprot Description

CCL13: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. By IL1/interleukin-1 and TNF. Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Chemokine; Motility/polarity/chemotaxis

Cellular Component: extracellular space; cell soma; rough endoplasmic reticulum; perinuclear region of cytoplasm; endocytic vesicle; dendrite; cytoplasm; extracellular region; perikaryon; synapse; nerve terminal

Molecular Function: heparin binding; G-protein-coupled receptor binding; CCR2 chemokine receptor binding; chemokine activity

Biological Process: positive regulation of cell adhesion; maternal process involved in pregnancy; positive regulation of leukocyte migration; response to glucocorticoid stimulus; response to lipopolysaccharide; response to antibiotic; monocyte chemotaxis; regulation of cell shape; response to vitamin B3; transforming growth factor beta receptor signaling pathway; negative regulation of neuron apoptosis; response to drug; neutrophil chemotaxis; organ regeneration; response to amino acid stimulus; positive regulation of tumor necrosis factor production; response to ethanol; response to bacterium; cellular response to insulin stimulus; response to heat; response to mechanical stimulus; positive regulation of endothelial cell proliferation; response to activity; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of collagen biosynthetic process; chemokinesis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; positive regulation of cell-cell adhesion; response to wounding; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; response to gamma radiation; inflammatory response; lymphocyte chemotaxis; aging; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; glial cell migration; macrophage chemotaxis; leukocyte migration during inflammatory response; cellular calcium ion homeostasis; negative regulation of angiogenesis; positive regulation of leukocyte mediated cytotoxicity; maternal process involved in parturition; response to hypoxia; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; astrocyte cell migration