Human MIG CLIA Kit | MIG clia kit

MIG (Human) LumiAb ELISA Kit

Cat. Num. AAA9510805

• Gene Names: CXCL9; CMK; MIG; Humig; SCYB9; crg-10
• Reactivity: Human
• Synonyms: MIG; MIG (Human) LumiAb ELISA Kit; Human MIG; C-X-C motif chemLumine 9; Gamma-interferon-induced monLumine; MonLumine induced by interferon-gamma; SCYB9; CMK; MIG clia kit

• Reactivity: Human
• Specificity: The LumiAb Human Midkine ELISA is capable of recognizing both recombinant and naturally produced Human Midkine proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.
• Sequence Length: 125

• Assay Type: Quantitative Sandwich
• Sensitivity: 8 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MIG clia kit

Principle of the Assay: The LumiAb Human Midkine Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Midkine concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Midkine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human Midkine, also known as Amphiregulin-Associated Protein, is a 143 amino acid cytokine protein encoded by the MDK gene located at locus 11p11.2 on chromosome 11. After initial synthesis, the protein undergoes proteolytic processing to remove the 20 residue signal sequence from the 123 residue Midkine peptide, allowing the protein to properly fold and mature. Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis that promotes angiogenesis, cell growth, and cell migration. Human Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis. Belonging to the pleiotrophin family, Midkine is also involved in early fetal adrenal gland development as well as neointima formation after arterial injury, possibly by mediating leukocyte recruitment.

Product Categories/Family for MIG clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 4505187
• NCBI GeneID: 4283
• NCBI Accession #: NP_002407.1
• NCBI GenBank Nucleotide #: NM_002416.2
• UniProt Accession #: Q07325
• NCBI Official Full Name: C-X-C motif chemokine 9
• NCBI Official Synonym Full Names: C-X-C motif chemokine ligand 9
• NCBI Official Symbol: CXCL9
• NCBI Official Synonym Symbols: CMK; MIG; Humig; SCYB9; crg-10
• NCBI Protein Information: C-X-C motif chemokine 9
• UniProt Protein Name: C-X-C motif chemokine 9
• Protein Family: Migration and invasion enhancer
• UniProt Gene Name: CXCL9
• UniProt Synonym Gene Names: CMK; MIG; SCYB9; HuMIG; MIG
• UniProt Entry Name: CXCL9_HUMAN

NCBI Description

This antimicrobial gene encodes a protein thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL9: Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: protein binding; CXCR3 chemokine receptor binding; chemokine activity; cytokine activity

Biological Process: defense response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; chemotaxis; signal transduction; positive regulation of myoblast differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; cell-cell signaling; cellular defense response; immune response; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; defense response to virus