Human I-TAC CLIA Kit | I-TAC clia kit

I-TAC (Human) LumiAb ELISA Kit

Cat. Num. AAA9510800

• Gene Names: CXCL11; IP9; H174; IP-9; b-R1; I-TAC; SCYB11; SCYB9B
• Reactivity: Human
• Synonyms: I-TAC; I-TAC (Human) LumiAb ELISA Kit; Human I-TAC; C-X-C motif chemLumine 11; Beta-R1; H174; Interferon gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; Small-inducible cytLumine B11; SCYB11; SCYB9B; I-TAC clia kit

• Reactivity: Human
• Specificity: The LumiAb Human IP-10 ELISA is capable of recognizing both recombinant and naturally produced Human IP-10 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 94

• Assay Type: Quantitative Sandwich
• Sensitivity: 8 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for I-TAC clia kit

Principle of the Assay: The LumiAb Human IP-10 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human IP-10 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human IP-10 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human IP-10, most commonly known as the C-X-C Motif Chemokine 10 or Interferon-gamma Induced Protein 10 is an 8.7 kDa protein consisting of 98 residues after initial expression from the gene CXCL10 at locus 4q21 on chromosome 4. The overall structural composition of IP-10 includes 4 beta strands ranging from 3 to 7 residues each connected by beta turns, followed by a 9 residue helix towards the C-terminus of the cytokine. After post translational processing, the 21 residue signal sequence is removed from the actual 77 residue IP-10 protein via proteolytic cleavage. Within the IP-10 protein, the CXCL-10 (1-73) peptide is produced by proteolytic cleavage after secretion from keratinocytes. IP-10 is known to be a chemokine of the C-X-C subfamily and ligand for the receptor CXCR3. High affinity binding with the cell surface receptor CXCR3 results in pleiotropic effects, including chemotactic stimulation of monocytes and macrophages, natural killer and T-cell migration, as well as modulation of adhesion molecule expression, inhibition of bone marrow colony formation and angiogenesis. IP-10 has been implicated as a key mediator in the Interferon-gamma (IFN-gamma) response as its expression is able to be rapidly increased by a variety of cell types when exposed to IFN-gamma.

Product Categories/Family for I-TAC clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 7674360
• NCBI GeneID: 6373
• UniProt Accession #: O14625
• NCBI Official Full Name: C-X-C motif chemokine 11
• NCBI Official Synonym Full Names: C-X-C motif chemokine ligand 11
• NCBI Official Symbol: CXCL11
• NCBI Official Synonym Symbols: IP9; H174; IP-9; b-R1; I-TAC; SCYB11; SCYB9B
• NCBI Protein Information: C-X-C motif chemokine 11
• UniProt Protein Name: C-X-C motif chemokine 11
• UniProt Gene Name: CXCL11
• UniProt Synonym Gene Names: ITAC; SCYB11; SCYB9B; IP-9; I-TAC
• UniProt Entry Name: CXL11_HUMAN

NCBI Description

Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC. This antimicrobial gene is a CXC member of the chemokine superfamily. Its encoded protein induces a chemotactic response in activated T-cells and is the dominant ligand for CXC receptor-3. The gene encoding this protein contains 4 exons and at least three polyadenylation signals which might reflect cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

Uniprot Description

CXCL11: Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Chemokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q21.2

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; CXCR3 chemokine receptor binding; chemokine activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; immune response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of cAMP metabolic process; signal transduction; chemotaxis; inflammatory response; regulation of cell proliferation