Mouse MDC CLIA Kit | MDC clia kit

MDC (Murine) LumiAb ELISA Kit

Cat. Num. AAA9510848

• Gene Names: Ccl22; MDC; DCBCK; ABCD-1; Scya22
• Reactivity: Mouse
• Synonyms: MDC; MDC (Murine) LumiAb ELISA Kit; Murine MDC; C-C motif chemLumine 22; Activated B and dendritic cell-derived; CC chemLumine ABCD-1; Small-inducible cytLumine A22; Abcd1; Scya22; MDC clia kit

• Reactivity: Mouse
• Specificity: The LumiAb Murine M-CSF ELISA is capable of recognizing both recombinant and naturally produced Murine M-CSF proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.
• Sequence Length: 92

• Assay Type: Quantitative Sandwich
• Sensitivity: 4 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MDC clia kit

Principle of the Assay: The LumiAb Murine M-CSF Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine M-CSF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Murine M-CSF while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human M-CSF, also known as Macrophage Colony-Stimulating Factor 1, is a 552 amino acid cytokine protein encoded by the Csf1 gene located on chromosome 3. After initial synthesis and translocation, the 32 residue signal sequence is proteolytically cleaved from the N-terminal of the peptide, allowing for the proper folding and maturation of M-CSF. Granulocyte or macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF induces cells of the monocyte or macrophage lineage and plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. The peptide can be N-glycosylated while the predominant soluble form is a chondroitin sulfate-containing proteoglycan. A defect in Csf1 is the cause of osteopetrosis. Osteopetrotic mice (op/op) are severely deficient in mature macrophages and osteoclasts, display failed tooth eruption, and have a restricted capacity for bone remodeling.

Product Categories/Family for MDC clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 6677879
• NCBI GeneID: 20299
• NCBI Accession #: NP_033163.1
• NCBI GenBank Nucleotide #: NM_009137.2
• UniProt Accession #: O88430
• NCBI Official Full Name: C-C motif chemokine 22
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 22
• NCBI Official Symbol: Ccl22
• NCBI Official Synonym Symbols: MDC; DCBCK; ABCD-1; Scya22
• NCBI Protein Information: C-C motif chemokine 22
• UniProt Protein Name: C-C motif chemokine 22
• UniProt Gene Name: Ccl22
• UniProt Synonym Gene Names: Abcd1; Scya22
• UniProt Entry Name: CCL22_MOUSE

Uniprot Description

CCL22: May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; cytokine activity

Biological Process: immune response; inflammatory response; chemotaxis