Human Mesothelin CLIA Kit | MSLN clia kit

Mesothelin (Human) LumiAb ELISA Kit

Cat. Num. AAA9510990

• Gene Names: MSLN; MPF; SMRP
• Reactivity: Human
• Synonyms: Mesothelin; Mesothelin (Human) LumiAb ELISA Kit; Human Mesothelin; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; MPF; MSLN clia kit

• Reactivity: Human
• Specificity: The LumiAb Human MCP-1 ELISA is capable of recognizing both recombinant and naturally produced Human MCP-1 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Sequence Length: 622

• Assay Type: Quantitative Sandwich
• Sensitivity: 4 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MSLN clia kit

Principle of the Assay: The LumiAb Human MCP-1 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human MCP-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human MCP-1 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Human MCP-1 or Monocyte Chemoattractant Protein-1, also known as C-C Motif Chemokine 2, is a 99 amino acid chemokine protein encoded by the CCL2 gene located at locus 17q11.2-17q12 on chromosome 17. MCP-1 is mainly a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Typically, the chemokine is believed to augment monocyte anti-tumor activity and has also been implicated in the pathogenesis of disease characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. Moreover, MCP-1 may also be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Able to exist as monomeric or homodimeric in equilibrium, MCP-1 can bind to CCR2 and CCR4, as well as being tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. A common post-translational modification that takes place with MCP-1 is processing at the N-terminus which can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophils to an eosinophil chemoattractant.

Product Categories/Family for MSLN clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 53988378
• NCBI GeneID: 10232
• NCBI Accession #: NP_005814.2
• NCBI GenBank Nucleotide #: NM_005823.6
• UniProt Accession #: Q13421
• NCBI Official Full Name: mesothelin isoform 1 preproprotein
• NCBI Official Synonym Full Names: mesothelin
• NCBI Official Symbol: MSLN
• NCBI Official Synonym Symbols: MPF; SMRP
• NCBI Protein Information: mesothelin
• UniProt Protein Name: Mesothelin
• Protein Family: Mesothelin
• UniProt Gene Name: MSLN
• UniProt Synonym Gene Names: MPF; MPF
• UniProt Entry Name: MSLN_HUMAN

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

Uniprot Description

MSLN: Membrane-anchored forms may play a role in cellular adhesion. Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. Belongs to the mesothelin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Cell adhesion

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; plasma membrane

Molecular Function: protein binding

Biological Process: pancreas development; cell adhesion