Immunohistochemistry (IHC)
(Anti-HDAC1 antibody IHC of human prostate carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)
Rabbit anti-Human HDAC1 Polyclonal Antibody | anti-HDAC1 antibody
Rabbit Polyclonal to Human HDAC1
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibo...
Immunohistochemistry (IHC)
(Anti-HDAC1 antibody IHC of human prostate carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)
Immunohistochemistry (IHC)
(Anti-HDAC1 antibody IHC of human prostate carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)
Immunofluorescence (IF)
(Anti-HDAC-1 polyclonal antibody-Immunofluorescence Microscopy. polyclonal Anti-HDAC-1 antibody () was used with Atto 425 Anti-Rabbit IgG (shown in red) for to detect HDAC-1 by immunofluorescence. Anti-Keratin monoclonal antibody was used with Dylight 488 goat anti-mouse shown in green) to detect Keratin by Immunofluorescence. Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems) equipped with a DFC 350FX Camera allowing sequential acquisition in wide-field, confocal and STED CW imaging modes and provided courtesy of: Myriam Gastard, PhD, personal communication, Leica Microsystems, Inc. USA.)
Immunofluorescence (IF)
(Anti-HDAC-1 polyclonal antibody-Immunofluorescence Microscopy. polyclonal Anti-HDAC-1 antibody () was used with Atto 425 Anti-Rabbit IgG (shown in red) for to detect HDAC-1 by immunofluorescence. Anti-Keratin monoclonal antibody was used with Dylight 488 goat anti-mouse shown in green) to detect Keratin by Immunofluorescence. Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems) equipped with a DFC 350FX Camera allowing sequential acquisition in wide-field, confocal and STED CW imaging modes and provided courtesy of: Myriam Gastard, PhD, personal communication, Leica Microsystems, Inc. USA.)
Immunofluorescence (IF)
(Immunofluorescence Microscopy - HDAC Antibody. Histone deacetylase (HDAC) antibody detects HDAC (colored GREEN) as used in STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 ug/mL final concentration of anti-HDAC and detected with 0.2 ug/mL DyLight488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody. Also shown in this 2-color STED image is an anti-tubulin monoclonal antibody [MOUSE] (detected with ATTO 425 conjugated anti-MOUSE IgG [GOAT] (secondary antibody (colored RED).)
Immunofluorescence (IF)
(Immunofluorescence Microscopy - HDAC Antibody. Histone deacetylase (HDAC) antibody detects HDAC (colored GREEN) as used in STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 ug/mL final concentration of anti-HDAC and detected with 0.2 ug/mL DyLight488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody. Also shown in this 2-color STED image is an anti-tubulin monoclonal antibody [MOUSE] (detected with ATTO 425 conjugated anti-MOUSE IgG [GOAT] (secondary antibody (colored RED).)
Western Blot (WB)
(Anti-HDAC-1 Antibody - Western Blot. Western blot of Affinity Purified anti-HDAC-1 antibody shows detection of a band at ~65 kD corresponding to human HDAC1 present in a 293 whole cell lysate (arrowhead). Approximately 35 ug of lysate was separated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1350. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
Western Blot (WB)
(Anti-HDAC-1 Antibody - Western Blot. Western blot of Affinity Purified anti-HDAC-1 antibody shows detection of a band at ~65 kD corresponding to human HDAC1 present in a 293 whole cell lysate (arrowhead). Approximately 35 ug of lysate was separated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1350. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
Western Blot (WB)
(Anti-HDAC-1 Antibody - Western Blot. p/n was used for Western blot of LNCaP prostate cancer cells. Lysate was loaded at 50 ug per lane and incubated with 1:1000 dilution of primary Ab (Lot. 16248). A band was detected at the expected molecular weight of 55 Kda. Personal communication Flavio Rizzolio, Temple University.)
Western Blot (WB)
(Anti-HDAC-1 Antibody - Western Blot. p/n was used for Western blot of LNCaP prostate cancer cells. Lysate was loaded at 50 ug per lane and incubated with 1:1000 dilution of primary Ab (Lot. 16248). A band was detected at the expected molecular weight of 55 Kda. Personal communication Flavio Rizzolio, Temple University.)
NCBI and Uniprot Product Information
NCBI Description
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
Uniprot Description
HDAC1: a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events.
Protein type: Nuclear receptor co-regulator; EC 3.5.1.98; Hydrolase
Chromosomal Location of Human Ortholog: 1p34
Cellular Component: nucleoplasm; protein complex; Sin3 complex; cytoplasm; histone deacetylase complex; nuclear chromatin; NuRD complex; chromatin; cytosol; nucleus
Molecular Function: deacetylase activity; nucleosomal DNA binding; transcription activator binding; histone deacetylase binding; protein deacetylase activity; protein N-terminus binding; transcription factor binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor activity
Biological Process: establishment and/or maintenance of chromatin architecture; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of cell cycle; protein amino acid deacetylation; negative regulation of gene expression, epigenetic; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; circadian regulation of gene expression; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; histone deacetylation; chromatin modification; odontogenesis of dentine-containing teeth; chromatin remodeling; ATP-dependent chromatin remodeling; epidermal cell differentiation; gene expression; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; embryonic digit morphogenesis; blood coagulation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
Research Articles on HDAC1
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Product Notes
The HDAC1 hdac1 (Catalog #AAA240605) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human HDAC1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA). ELISA (1:10000 - 1:50000), IF (1:200 - 1:1000), IHC-P (20 ug/ml), WB (1:1000 - 1:5000) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibo.. Researchers should empirically determine the suitability of the HDAC1 hdac1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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