Rabbit ZIF268 / EGR1 Polyclonal Antibody | anti-ZIF268 antibody

Rabbit Polyclonal to Human ZIF268 / EGR1

Cat. Num. AAA240672

• Gene Names: EGR1; TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268
• Reactivity: Chimpanzee, Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: ZIF268 / EGR1; Polyclonal Antibody; Rabbit Polyclonal to Human ZIF268 / EGR1; Anti-ZIF268 / EGR1 Antibody (aa94-108) IHC-plus; EGR1; AT225; Early growth response 1; G0S30; KROX24; NGFIA; KROX-24; TIS8; ZIF-268; ZIF268; Zinc finger protein Krox-24; EGR-1; NGFI-A; Transcription factor ETR103; Transcription factor Zif268; Zinc finger protein 225; Human ZIF268; anti-ZIF268 antibody

• Host: Rabbit
• Reactivity: Chimpanzee, Human, Mouse
• Clonality: Polyclonal
• Specificity: Amino acids 94-108 of Human EGR-1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 0.93 mg/ml (varies by lot)
• Sequence Length: 543

• Applicable Applications for anti-ZIF268 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:4000 - 1:16000), IHC-P (10 ug/ml), WB (1:500 - 1:3000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibo...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human EGR1.
• Immunogen Type: Synthetic peptide
• Immunogen: ZIF268 / EGR1 antibody was raised against synthetic peptide from human EGR1.
• Antigen Modification: aa94-108
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-EGR1 antibody IHC of human lung small cell carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Anti-EGR-1 Antibody - Western Blot. Western blot of Affinity Purified anti-EGR-1 antibody shows detection of a predominant band at ~58 kD corresponding to EGR-1 present in mouse embryonic fibroblast whole cell lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1500. Reaction occurred 2 hours at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)

Related Product Information for anti-ZIF268 antibody

EGR-1 (also called Early Growth Response protein 1, Krox-24 protein, ZIF268, Nerve growth factor-induced protein A or NGFI-A, Transcription factor ETR103, and Zinc finger protein 225 or AT225) is a transcriptional regulator that recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site). EGR1 has been implicated in diverse roles in the regulation of cell growth, apoptosis, and differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 4503493
• NCBI GeneID: 1958
• NCBI Accession #: NP_001955.1
• NCBI GenBank Nucleotide #: NM_001964.2
• UniProt Accession #: P18146
• Molecular Weight: 57,507 Da
• NCBI Official Full Name: early growth response protein 1
• NCBI Official Synonym Full Names: early growth response 1
• NCBI Official Symbol: EGR1
• NCBI Official Synonym Symbols: TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268
• NCBI Protein Information: early growth response protein 1; EGR-1; zinc finger protein 225; transcription factor ETR103; transcription factor Zif268; zinc finger protein Krox-24; nerve growth factor-induced protein A
• UniProt Protein Name: Early growth response protein 1
• UniProt Gene Name: EGR1
• UniProt Synonym Gene Names: KROX24; ZNF225; EGR-1; NGFI-A
• UniProt Entry Name: EGR1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene. [provided by RefSeq, Dec 2014]

Uniprot Description

EGR1: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. By growth factors. Belongs to the EGR C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: histone acetyltransferase binding; protein binding; DNA binding; sequence-specific DNA binding; metal ion binding; double-stranded DNA binding; transcription factor activity; transcription factor binding

Biological Process: response to nutrient levels; regulation of long-term neuronal synaptic plasticity; circadian rhythm; transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; response to amphetamine; negative regulation of transcription from RNA polymerase II promoter; response to cocaine; BMP signaling pathway; learning and/or memory; response to ethanol; cellular response to insulin stimulus; positive regulation of neuron apoptosis; response to glucose stimulus; oligodendrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; response to electrical stimulus; regulation of protein sumoylation; T cell differentiation; negative regulation of apoptosis