Rabbit S100 Protein Polyclonal Antibody

Rabbit Polyclonal to Bovine S100 Protein

Cat. Num. AAA240461

• Reactivity: Bovine, Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: S100 Protein; Polyclonal Antibody; Rabbit Polyclonal to Bovine S100 Protein; Anti-S100 Protein Antibody IHC-plus; Bovine S100 Protein; anti-S100 Protein antibody

• Host: Rabbit
• Reactivity: Bovine, Human
• Clonality: Polyclonal
• Specificity: Full-length bovine S100 protein (mixture of aa homodimers and ab heterodimers).
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-S100 Protein antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000 - 1:20000), IHC-P (2.5 ug/ml), WB (1:500 - 1:3000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibo...
• Target Species: Bovine
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-S100 Protein antibody IHC of human prostate, nerve. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-S-100 Antibody - Western Blot. Western blot of Affinity Purified anti-S-100 antibody shows detection of a band ~11 kD corresponding to bovine S-100 monomer (100 ng loaded, arrowhead lane 1). The antibody also detects S-100 from rat brain lysate (lane 2). Approximately 35 ug of a rat brain whole cell lysate was separated by 16% SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1000 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)