Rabbit anti-Human ZNF828 Polyclonal Antibody | anti-ZNF828 antibody

ZNF828, ID (CHAMP1, C13orf8, CAMP, KIAA1802, ZNF828, Chromosome alignment-maintaining phosphoprotein 1, Zinc finger protein 828) (FITC)

Cat. Num. AAA6367837

• Gene Names: CHAMP1; CAMP; CHAMP; ZNF828; C13orf8
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZNF828; Polyclonal Antibody; ID (CHAMP1; C13orf8; CAMP; KIAA1802; Chromosome alignment-maintaining phosphoprotein 1; Zinc finger protein 828) (FITC); CHAMP1; Zinc finger protein 828; anti-ZNF828 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ZNF828 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ZNF828 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-229 amino acids from the Central region of human ZNF828.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ZNF828 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ZNF828 antibody detected the ZNF828 protein (arrow).)

Product Categories/Family for anti-ZNF828 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Itoh, G., et al. EMBO J. (2010). In press: Olsen, J.V., et al. Cell 127(3):635-648(2006). Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004). Dunham, A., et al. Nature 428(6982):522-528(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 255918139
• NCBI GeneID: 283489
• NCBI Accession #: NP_001157616.1
• NCBI GenBank Nucleotide #: NM_001164144.1
• UniProt Accession #: Q96JM3; Q6P181; Q8NC88; Q9BST0; B3KU06
• Molecular Weight: 89,099 Da
• NCBI Official Full Name: chromosome alignment-maintaining phosphoprotein 1
• NCBI Official Synonym Full Names: chromosome alignment maintaining phosphoprotein 1
• NCBI Official Symbol: CHAMP1
• NCBI Official Synonym Symbols: CAMP; CHAMP; ZNF828; C13orf8
• NCBI Protein Information: chromosome alignment-maintaining phosphoprotein 1; zinc finger protein 828
• UniProt Protein Name: Chromosome alignment-maintaining phosphoprotein 1
• UniProt Gene Name: CHAMP1
• UniProt Synonym Gene Names: C13orf8; CAMP; CHAMP; KIAA1802; ZNF828
• UniProt Entry Name: CHAP1_HUMAN

Uniprot Description

ZNF828: Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.

Protein type: RNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: nucleoplasm; cytoplasm; condensed chromosome; spindle; nucleus

Molecular Function: protein binding; nucleic acid binding; metal ion binding

Biological Process: sister chromatid biorientation; attachment of spindle microtubules to kinetochore during mitosis