Rabbit FANCG Polyclonal Antibody | anti-FANCG antibody

Rabbit Polyclonal to Human FANCG

Cat. Num. AAA240574

• Gene Names: FANCG; FAG; XRCC9
• Reactivity: Chimpanzee, Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: FANCG; Polyclonal Antibody; Rabbit Polyclonal to Human FANCG; Anti-FANCG Antibody (aa1-12) IHC-plus; DNA repair protein XRCC9; Fanconi anemia group G protein; FAG; Protein FACG; XRCC9; Human FANCG; anti-FANCG antibody

• Host: Rabbit
• Reactivity: Chimpanzee, Human
• Clonality: Polyclonal
• Specificity: Amino acids 1-12 of human FANCG protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 0.93 mg/ml (varies by lot)
• Sequence Length: 622

• Applicable Applications for anti-FANCG antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000 - 1:50000), IF, IHC-P (2.5 ug/ml), WB (1:500 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human FANCG.
• Immunogen Type: Synthetic peptide
• Immunogen: FANCG antibody was raised against synthetic peptide from human FANCG.
• Antigen Modification: aa1-12
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-FANCG antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-FANCG antibody - Western Blot. Western blot of affinity purified anti-FANCG antibody shows detection of a band at ~69 kD (arrowhead) corresponding to FANCG present in a HeLa whole cell lysate. Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4C with the primary antibody diluted to 1:500. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)

Related Product Information for anti-FANCG antibody

FANCG, involved in Fanconi anemia, confers resistance to both hygromycin and mitomycin C. FANCG contains a 5-prime GC-rich untranslated region characteristic of housekeeping genes. The putative 622-amino acid protein has a leucine-zipper motif at its N-terminus. Fanconi anemia is an autosomal recessive disorder with diverse clinical symptoms, including developmental anomalies, bone marrow failure, and early occurrence of malignancies. A minimum of 8 FA genes have been identified.

NCBI and Uniprot Product Information

• NCBI GI #: 4759336
• NCBI GeneID: 2189
• NCBI Accession #: NP_004620.1
• NCBI GenBank Nucleotide #: NM_004629.1
• UniProt Accession #: O15287
• Molecular Weight: 68,554 Da
• NCBI Official Full Name: Fanconi anemia group G protein
• NCBI Official Synonym Full Names: Fanconi anemia, complementation group G
• NCBI Official Symbol: FANCG
• NCBI Official Synonym Symbols: FAG; XRCC9
• NCBI Protein Information: Fanconi anemia group G protein; DNA repair protein XRCC9; X-ray repair, complementing defective, in Chinese hamster, 9; X-ray repair complementing defective repair in Chinese hamster cells 9
• UniProt Protein Name: Fanconi anemia group G protein
• Protein Family: Fanconi anemia group G protein
• UniProt Gene Name: FANCG
• UniProt Synonym Gene Names: XRCC9; Protein FACG
• UniProt Entry Name: FANCG_HUMAN

NCBI Description

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G. [provided by RefSeq, Jul 2008]

Uniprot Description

FANCG: DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene. Defects in FANCG are a cause of Fanconi anemia complementation group G (FANCG). A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; plasma membrane; nucleolus

Molecular Function: protein binding; damaged DNA binding

Biological Process: mitochondrion organization and biogenesis; response to radiation; ovarian follicle development; DNA repair; cell cycle checkpoint; spermatid development

Disease: Fanconi Anemia, Complementation Group G; Tracheoesophageal Fistula With Or Without Esophageal Atresia