Mouse TUBA1B Monoclonal Antibody | anti-TUBA1B antibody

Mouse Monoclonal [clone 17H11.F10] (IgG) to Human TUBA1B

Cat. Num. AAA249064

• Gene Names: TUBA1B; K-ALPHA-1
• Reactivity: Bovine, Chicken, Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: TUBA1B; Monoclonal Antibody; Mouse Monoclonal [clone 17H11.F10] (IgG) to Human TUBA1B; Anti-TUBA1B Antibody (C-Terminus; clone 17H11.F10) IHC-plus; Alpha-tubulin ubiquitous; Alpha tubulin; K-ALPHA-1; Tubulin; alpha 1b; Tubulin alpha-ubiquitous chain; alpha; ubiquitous; Tubulin K-alpha-1; Tubulin alpha chain; Tubulin alpha-1B chain; Human TUBA1B; anti-TUBA1B antibody

• Host: Mouse
• Reactivity: Bovine, Chicken, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 17H11.F10
• Specificity: Anti-Tubulin Loading Control Antibody was purified Protein A chromatography. This Loading Control antibody is directed against alpha tubulin. A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
• Purity/Purification: Protein A Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 451

• Applicable Applications for anti-TUBA1B antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:300000), IF (0.1 ug/ml), IHC-P (5 ug/ml), WB (1:1000); Usage: Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kD in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.
• Target Species: Human
• Immunogen Description: Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.
• Immunogen Type: Synthetic peptide
• Immunogen: TUBA1B antibody was raised against anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-TUBA1B antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-alpha-Tubulin Monoclonal Antibody - Immunohistochemistry. anti-a-tubulin monoclonal antibody was used at a 2.5 ug/mL to detect tubulin in thyroid follicular epithelium (40X) showing moderate to strong cytoplasmic staining (image). Moderate to strong cytoplasmic staining was also observed within subsets of neurons and glia, and epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. tissue was formalin-fixed and paraffin embedded.)

Immunofluorescence (IF)

(Anti-tubulin Monoclonal antibody-Immunofluorescence. mouse monoclonal anti-tubulin antibody was used with ATTO 425 Goat anti-mouse (shown in red) to detect tubulin by immunofluorescence. Dylight 488 Goat anti-Rabbit (shown in green) was used in the same experiment to detect rabbit anti-Clathrin polyclonal antibody Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems) equipped with a DFC 350FX Camera allowing sequential acquisition in wide-field, confocal and STED CW imaging modes and provided courtesy of: Myriam Gastard, PhD, personal communication, Leica Microsystems, Inc. USA.)

Immunofluorescence (IF)

(Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - Immunofluorescence. Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody Immunofluorescence image showing MCF-7 cell staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody in green and staining of Anti-Gli-3 (RABBIT) Antibody in red.)

Immunofluorescence (IF)

(Immunofluorescence Microscopy - alpha-Tubulin Monoclonal Antibody -. a-tubulin monoclonal antibody detects tubulin (colored RED) in STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 ug/mL final concentration of anti-a-tubulin and detected with 0.2 ug/mL ATTO 425 conjugated anti-MOUSE IgG [GOAT] (secondary antibody. Also shown in this 2-color STED image is Anti-HDAC-1 [RABBIT] detected with DyLight488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody (colored GREEN). Image courtesy of Myriam Gastard, Leica Microsystems, USA.)

Immunofluorescence (IF)

(Anti-alpha-Tubulin Monoclonal Antibody - Immunofluorescence Microscopy. anti-a-tubulin monoclonal antibody was used at a 0.1 ug/mL to detect tubulin in 4% paraformaldehyde fixed A459 cells. Staining is shown using conventional confocal microscopy (left panel) and by high resolution TCS STED nanoscopy (right panel). DyLight488 conjugated anti-mouse IgG secondary antibody (p/n was used for detection at 1 ug/mL. Personal Communication, Myriam. Gastard, Leica Microsystems, Exton PA.)

Immunofluorescence (IF)

(Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody - Immunofluorescence. Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody Immunofluorescence image one showing MCF-7 cell staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody in green. Immunofluorescence image two showing MCF-7 cell staining of Anti-Gli-3 (RABBIT) Antibody in red. Immunofluorescence image three showing MCF-7 cell superimposed staining of Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody in green and staining of Anti-Gli-3 (RABBIT) antibody in red.)

Western Blot (WB)

(Anti-alpha-Tubulin Monoclonal Antibody - Western Blot. HeLa whole cell lysate (left lane) and HEK293 whole cell lysate (right lane) were loaded with 10 ug of lysate each. The blot was blocked with Blocking Buffer (MB-070) for 30 min at RT, then washed and incubated with anti-Tubulin monoclonal antibody diluted in Blocking Buffer (p/n MB-070) at 1:1000 for 1 h at RT. After washing, blot was incubated with a 1:40000 dilution of HRP Rb a-Ms IgG secondary antibody in Blocking Buffer (p/n MB-070) for 30 minutes at RT. Data was collected using Bio-Rad VersaDoc 4000 MP.)

NCBI and Uniprot Product Information

• NCBI GI #: 57013276
• NCBI GeneID: 10376
• NCBI Accession #: NP_006073.2
• NCBI GenBank Nucleotide #: NM_006082.2
• UniProt Accession #: P68363; P04687; P05209; Q27I68; Q8WU19
• Molecular Weight: 37,218 Da
• NCBI Official Full Name: tubulin alpha-1B chain
• NCBI Official Synonym Full Names: tubulin, alpha 1b
• NCBI Official Symbol: TUBA1B
• NCBI Official Synonym Symbols: K-ALPHA-1
• NCBI Protein Information: tubulin alpha-1B chain; alpha tubulin; tubulin K-alpha-1; alpha-tubulin ubiquitous; tubulin, alpha, ubiquitous; tubulin alpha-ubiquitous chain
• UniProt Protein Name: Tubulin alpha-1B chain
• UniProt Gene Name: TUBA1B
• UniProt Entry Name: TBA1B_HUMAN

Uniprot Description

TUBA1B: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: microtubule; cytoplasmic microtubule

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; double-stranded RNA binding; structural molecule activity

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; cell division; cytoskeleton-dependent intracellular transport; microtubule cytoskeleton organization and biogenesis; microtubule-based process