Rabbit anti-Mouse, Rat S100-A8/MRP8 Polyclonal Antibody | anti-S100-A8/MRP8 antibody

S100-A8/MRP8 Polyclonal Antibody

Cat. Num. AAA3015148

• Gene Names: S100a8; p8; B8Ag; CFAg; Caga; MRP8; CP-10; 60B8Ag; AI323541
• Reactivity: Mouse, Rat
• Applications: Flow Cytometry, Functional Assay
• Purity: affinity purified by Protein A
• Synonyms: S100-A8/MRP8; Polyclonal Antibody; S100-A8/MRP8 Polyclonal Antibody; 60B8Ag; AI323541; B8Ag; BEE11; CAGA; Calgranulin-A; Calprotectin L1L subunit; Calprotectin; included; CFAG; CGLA; Chemotactic cytokine CP-10; CP-10; Cystic fibrosis antigen; L1Ag; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP-8; Myeloid-related protein 8; Neutrophil cytosolic 7 kDa protein; NIF; p8; Pro-inflammatory S100 cytokine; Protein S100-A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium binding protein A8; S100 calcium-binding protein A8; S100A8; S100A8/S100A9 complex; S10A8_MOUSE; Urinary stone protein band A; anti-S100-A8/MRP8 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Specificity: S100-A8/MRP8 Polyclonal Antibody detects endogenous levels of S100-A8/MRP8 protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-S100-A8/MRP8 antibody: Flow Cytometry (FC/FACS/FCM)
• Application Notes: FC/FACS/FCM: 2ug/Test
• Immunogen: KLH conjugated synthetic peptide derived from mouse S100A8:25-89/89
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Testing Data

Related Product Information for anti-S100-A8/MRP8 antibody

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq].

NCBI and Uniprot Product Information

• NCBI GI #: 7305453
• NCBI GeneID: 20201
• NCBI Accession #: NP_038678.1
• NCBI GenBank Nucleotide #: NM_013650.2
• UniProt Accession #: P27005; P31724
• NCBI Official Full Name: protein S100-A8
• NCBI Official Synonym Full Names: S100 calcium binding protein A8 (calgranulin A)
• NCBI Official Symbol: S100a8
• NCBI Official Synonym Symbols: p8; B8Ag; CFAg; Caga; MRP8; CP-10; 60B8Ag; AI323541
• NCBI Protein Information: protein S100-A8; MRP-8; S100 calcium-binding protein A8; calgranulin-A; chemotactic S100 protein; chemotactic cytokine CP-10; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; pro-inflammatory S100 cytokine
• UniProt Protein Name: Protein S100-A8
• UniProt Gene Name: S100a8
• UniProt Synonym Gene Names: Caga; Mrp8; MRP-8; p8
• UniProt Entry Name: S10A8_MOUSE

Uniprot Description

S100A8: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Belongs to the S-100 family.

Cellular Component: extracellular space; cytoskeleton; membrane; cytoplasm; plasma membrane; extracellular region; nucleus

Molecular Function: arachidonic acid binding; antioxidant activity; RAGE receptor binding; zinc ion binding; microtubule binding; metal ion binding; calcium ion binding

Biological Process: caspase activation; neutrophil chemotaxis; apoptosis; immune system process; regulation of inflammatory response; innate immune response; autophagy; positive regulation of peptide secretion; chemotaxis; inflammatory response; leukocyte migration during inflammatory response; positive regulation of inflammatory response