Rabbit anti-Human, Mouse RIF1 Polyclonal Antibody | anti-RIF1 antibody

Rabbit Polyclonal to Mouse RIF1

Cat. Num. AAA241365

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: RIF1; Polyclonal Antibody; Rabbit Polyclonal to Mouse RIF1; Anti-RIF1 Antibody (aa2406-2419) IHC-plus; Mouse RIF1; anti-RIF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Amino acids 2406-2419 of Mouse Rif1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 2472

• Applicable Applications for anti-RIF1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000 - 1:40000), IHC-P (5 ug/ml), WB (1:500 - 1:3000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Mouse
• Immunogen Description: Synthetic peptide from mouse RIF1.
• Immunogen Type: Synthetic peptide
• Immunogen: RIF1 antibody was raised against synthetic peptide from mouse RIF1.
• Antigen Modification: aa2406-2419
• Preparation and Storage: Store vial at -20 degree C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing.

Immunohistochemistry (IHC)

(Anti-RIF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-RIF1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-Rif1 Antibody - Western Blot. Western blot of Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kD corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 ug of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)

NCBI and Uniprot Product Information

• NCBI GI #: 56676335
• NCBI GeneID: 55183
• NCBI Accession #: NP_060621.3
• NCBI GenBank Nucleotide #: NM_018151.4
• UniProt Accession #: Q5UIP0; Q5H9R3; Q5UIP2; Q66YK6; Q6PRU2; Q8TE94; Q99772; Q9H830; Q9H9B9; Q9NVP5; A6NC27; C9JBR1
• Molecular Weight: 271,689 Da
• NCBI Official Full Name: telomere-associated protein RIF1 isoform 1
• NCBI Official Synonym Full Names: replication timing regulatory factor 1
• NCBI Official Symbol: RIF1
• NCBI Protein Information: telomere-associated protein RIF1; RAP1 interacting factor homolog; rap1-interacting factor 1 homolog
• UniProt Protein Name: Telomere-associated protein RIF1
• Protein Family: Telomere-associated protein
• UniProt Gene Name: RIF1
• UniProt Entry Name: RIF1_HUMAN

NCBI Description

This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Uniprot Description

RIF1: Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S- phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 2q23.3

Cellular Component: chromosome, telomeric region; male pronucleus; cytoplasm; plasma membrane; female pronucleus; spindle; nucleus

Biological Process: stem cell maintenance; cell cycle; response to DNA damage stimulus