Testing Data
Rabbit MMP-2 Polyclonal Antibody | anti-MMP-2 antibody
Rabbit anti MMP-2 Polyclonal antibody
Reactivity
Human, Rat, Mouse, Chicken, Canine
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by Epitope Affinity Purification.
Synonyms
MMP-2; Polyclonal Antibody; Rabbit anti MMP-2 Polyclonal antibody; Rabbit anti-MMP-2; Matrix Metalloproteinase 2 (MMP2); Gelatinase A; 72 kDa type IV collagenase precursor; anti-MMP-2 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Chicken, Canine
Clonality
Polyclonal
Specificity
This antibody recognizes ~62 and 72 kDa of human MMP2 protein. This antibody reacts with human, rat, mouse. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-MMP-2 antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
Antigen Preparation
A synthetic peptide corresponding to C-term of human MMP2. This sequence is identical among human, rat, mouse, chicken, dog and bovine.
Positive Control
Breast cancer
Cellular Location
Cytoplasmic and extracellular matrix
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C -8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-MMP-2 antibody
Matrix metalloproteinases (MMPs) belong to a family of proteinases that target many extracellular matrix proteins including additional proteinases, growth factors, cell surface receptors and adhesion molecules. MMPs contain common domain structures that include a signal sequence, a propeptide, a catalytic domain, and a hemopexin-like (Hpx) domain. The MMP activity requires proteolytic cleavage of MMPs in order to generate activie MMPs by release of the inhibitory propeptide domain from the whole molecules. MMP-2, MMP-3, MMP-7, MMP-9 and MMP-13 have been characterized as important factors for normal tissue remodeling during embryo development and wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis. MMP activities are correlated with cancer metastatic process.
References
Brigitte Lelongt, et al. Matrix Metalloproteinases MMP2 and MMP9 Are Produced in Early Stages of Kidney Morphogenesis but Only MMP9 Is Required for Renal Organogenesis In Vitro. J. Cell Biol. 1997. 136 (6), 1363-1373.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
62kDa, 72 kDa
NCBI Official Full Name
MMP-2
NCBI Official Synonym Full Names
matrix metallopeptidase 2
NCBI Official Symbol
Mmp2
NCBI Protein Information
72 kDa type IV collagenase; MMP-2; gelatinase A; 72 kDa gelatinase; matrix metalloproteinase 2; matrix metalloproteinase-2
UniProt Protein Name
MMP-2
UniProt Gene Name
Mmp2
UniProt Synonym Gene Names
MMP-2
UniProt Entry Name
Q9JHC2_RAT
NCBI Description
plays important roles in wound healing, angiogenesis, platelet aggregation, and tumor metastasis; mediates the response of the intrinsic glomerular mesangial cell to inflammatory stimuli [RGD]
Research Articles on MMP-2
Similar Products
Product Notes
The MMP-2 mmp2 (Catalog #AAA462081) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti MMP-2 Polyclonal antibody reacts with Human, Rat, Mouse, Chicken, Canine and may cross-react with other species as described in the data sheet. AAA Biotech's MMP-2 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 2-5 ug/ml. Researchers should empirically determine the suitability of the MMP-2 mmp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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