Rabbit CAMK2A / CaMKII Alpha Polyclonal Antibody | anti-CAMK2A antibody

Rabbit Polyclonal to Human CAMK2A / CaMKII Alpha

Cat. Num. AAA240611

• Gene Names: CAMK2A; CAMKA
• Reactivity: Dog, Human, Mouse, Orangutan, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Purified
• Synonyms: CAMK2A / CaMKII Alpha; Polyclonal Antibody; Rabbit Polyclonal to Human CAMK2A / CaMKII Alpha; Anti-CAMK2A / CaMKII Alpha Antibody (aa6-23) IHC-plus; CAMK2A; AlphaCaMKII; CaM-kinase II alpha chain; CaMK-II subunit alpha; CAMKA; CaM kinase II alpha; CaMK II alpha; CaMK-II alpha subunit; CaMKIINalpha; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaMKII alpha; CaMKIIalpha; KIAA0968; Human CAMK2A; CaMKII Alpha; anti-CAMK2A antibody

• Host: Rabbit
• Reactivity: Dog, Human, Mouse, Orangutan, Rat
• Clonality: Polyclonal
• Specificity: Amino acids 6-23 of Human CaM Kinase II protein.
• Purity/Purification: Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 489

• Applicable Applications for anti-CAMK2A antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:4000 - 1:20000), IF, IHC-P (2.5 ug/ml), WB (1:1000 - 1:3000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human CAMK2A.
• Immunogen Type: Synthetic peptide
• Immunogen: CAMK2A / CaMKII Alpha antibody was raised against synthetic peptide from human CAMK2A.
• Antigen Modification: aa6-23
• Preparation and Storage: Store vial at -20 degree C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CAMK2A antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-CaM Kinase II Antibody - Western Blot. Western blot of Affinity Purified anti-CaM Kinase II antibody shows detection of a band ~54 kD corresponding to human alpha CaM Kinase II (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 ug of a mouse brain whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase II was similarly detected on lysates from rat brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1500 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)

Related Product Information for anti-CAMK2A antibody

CAMK2Alpha, together with the beta, delta, and gamma subunits, constititute the CAMK2 heteromultimeric holoenzyme serine/threonine kinase. CAMK2A has been implicated in learning, where it potentiates synaptic transmission by phosphorylating AMPA receptors. Variants are produced by alternative splicing.

Product Categories/Family for anti-CAMK2A antibody

Family: Protein Kinase; Subfamily: CAMK2

NCBI and Uniprot Product Information

• NCBI GI #: 25952114
• NCBI GeneID: 815
• NCBI Accession #: NP_057065.2
• NCBI GenBank Nucleotide #: NM_015981.3
• UniProt Accession #: Q9UQM7; Q9UL21; Q9Y2H4; Q9Y352
• Molecular Weight: 55,320 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 1
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II alpha
• NCBI Official Symbol: CAMK2A
• NCBI Official Synonym Symbols: CAMKA
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit alpha; CaMKIINalpha; CaMK-II alpha subunit; caMK-II subunit alpha; CaM-kinase II alpha chain; CaM kinase II alpha subunit; caM kinase II subunit alpha; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit alpha
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK2A
• UniProt Synonym Gene Names: CAMKA; KIAA0968; CaM kinase II subunit alpha; CaMK-II subunit alpha
• UniProt Entry Name: KCC2A_HUMAN

NCBI Description

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]

Uniprot Description

CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, CAMK; Kinase, protein; EC 2.7.11.17; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: presynaptic membrane; nucleoplasm; mitochondrion; plasma membrane; cell junction; cytosol; nucleus

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; glutamate receptor binding; protein binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; kinase activity; ATP binding

Biological Process: synaptic transmission; peptidyl-serine phosphorylation; calcium ion transport; cytokine and chemokine mediated signaling pathway; protein amino acid autophosphorylation; regulation of neurotransmitter secretion; positive regulation of calcium ion transport; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle; regulation of neuronal synaptic plasticity; activation of NF-kappaB transcription factor