Rabbit GGA3 Polyclonal Antibody | anti-GGA3 antibody

Rabbit Polyclonal to Human GGA3

Cat. Num. AAA240568

• Reactivity: Chimpanzee, Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: GGA3; Polyclonal Antibody; Rabbit Polyclonal to Human GGA3; Anti-GGA3 Antibody (aa400-415) IHC-plus; KIAA0154; Human GGA3; anti-GGA3 antibody

• Host: Rabbit
• Reactivity: Chimpanzee, Human
• Clonality: Polyclonal
• Specificity: Amino acids 400-415 of Human GGA3.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 0.62 mg/ml (varies by lot)
• Sequence Length: 651

• Applicable Applications for anti-GGA3 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000 - 1:50000), IF, IHC-P (2.5 ug/ml), WB (1:500 - 1:3000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human GGA3.
• Immunogen Type: Synthetic peptide
• Immunogen: GGA3 antibody was raised against synthetic peptide from human GGA3.
• Antigen Modification: aa400-415
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-GGA3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-GGA3 Antibody - Western Blot. Western blot of Affinity Purified anti-GGA3 antibody shows detection of a band at ~110 kD corresponding to GFP-GGA3 fusion protein present in a lysate of HEK293 cells over- expressing the recombinant protein (lane 2, arrowhead). Pre-incubation of antibody with immunizing peptide blocks specific staining (lane 3). MW markers are shown in lane 1 (700 nm channel - red). Approximately 35 ug of lysate was separated on a 16% Tricine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:600. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (for 45 min at room temperature (800 nm channel - green). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)

NCBI and Uniprot Product Information

• NCBI GI #: 289577109
• NCBI GeneID: 23163
• NCBI Accession #: NP_001166174.1
• NCBI GenBank Nucleotide #: NM_001172703.2
• UniProt Accession #: Q9NZ52; Q15017; Q6IS16; Q9UJY3; B7Z7E2; B7Z7M9; J3KRN0
• Molecular Weight: 70,148 Da
• NCBI Official Full Name: ADP-ribosylation factor-binding protein GGA3 isoform 1
• NCBI Official Synonym Full Names: golgi-associated, gamma adaptin ear containing, ARF binding protein 3
• NCBI Official Symbol: GGA3
• NCBI Protein Information: ADP-ribosylation factor-binding protein GGA3; Golgi-localized, gamma ear-containing, ARF-binding protein 3
• UniProt Protein Name: ADP-ribosylation factor-binding protein GGA3
• Protein Family: ADP-ribosylation factor-binding protein
• UniProt Gene Name: GGA3
• UniProt Synonym Gene Names: KIAA0154
• UniProt Entry Name: GGA3_HUMAN

NCBI Description

This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010]

Uniprot Description

GGA3: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Monomer. Interacts with SORT1, SORL1, LRP3, GGA binding partner (GGABP) and P200. Interacts with GGA1 and GGA2. Binds to clathrin and activated ARFs. Binds RABEP1 and RABGEF1. Interacts with the membrane proteins M6PR/CD-MPR, IGF2R/CI-MPR and BACE1 and the accessory proteins SYNRG, EPN4, NECAP1, NECAP2 and AFTPH/aftiphilin. Interacts with TSG101 and UBC. Ubiquitously expressed. Belongs to the GGA protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: clathrin adaptor complex; endosome membrane; trans-Golgi network

Molecular Function: ADP-ribosylation factor binding; protein binding

Biological Process: vesicle-mediated transport; intracellular protein transport; positive regulation of protein catabolic process