SDS-Page
(Detection of Angiocidin. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/ml goat anti-mAngiocidin.)
Goat anti-Mouse Angiocidin Polyclonal Antibody
Angiocidin (S5a, RPN10a, Mcb1)
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Angiocidin; Polyclonal Antibody; Angiocidin (S5a; RPN10a; Mcb1); Anti -Angiocidin (S5a; anti-Angiocidin antibody
Host
Goat
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Angiocidin.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Applicable Applications for anti-Angiocidin antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Dilution: ELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml
Western Blot: 1.0ug/ml
Immunogen
Recombinant mouse Angiocidin isoform A aa2-254; O35226 (E. coli).
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of Angiocidin. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/ml goat anti-mAngiocidin.)
SDS-Page
(Detection of Angiocidin. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/ml goat anti-mAngiocidin.)
Related Product Information for anti-Angiocidin antibody
Angiocidin (also S5a, RPN10a and Mcb1) is a 41kD (predicted) member of the proteosome subunit S5A family of proteins. It runs anomalously in SDS-page at 55kD. Intracellularly, Angiocidin is a component of the 26S proteosome that degrades ubiquitinated proteins. Extracellularly, it inhibits angiogenesis by binding to collagen and beta 1 integrins. Mouse Angiocidin is 376aa in length. It contains a vWF-A domain (aa5-188), a beta 1 interacting site (aa86-105) and two ubiquitin-interacting motifs (aa211-230 and 282-301). There are four splice variants. Three show a GlyGluArg insertion after Glu254, with two of these showing an additional three aa substitution for aa255-376, and a 45aa substitution for aa321-376, respectfully. A fourth shows a 51aa substitution for aa299-376. Over aa1-254, mouse Angiocidin shares > 99% aa identity with rat and human Angiocidin.
Product Categories/Family for anti-Angiocidin antibody
Similar Products
Product Notes
The Angiocidin (Catalog #AAA621459) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Angiocidin (S5a, RPN10a, Mcb1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Angiocidin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml. Researchers should empirically determine the suitability of the Angiocidin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angiocidin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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