SDS-Page
(Detection of 15-PGDH. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/ml goat anti-h15-PGDH.)
Goat anti-Human Prostaglandin Dehydrogenase 1 Polyclonal Antibody
Prostaglandin Dehydrogenase 1 (HPGD, PGDH1, 15-PGDH, hydroxyprostaglandin dehydrogenase 15-(NAD), Prostaglandin dehydrogenase 1)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Prostaglandin Dehydrogenase 1; Polyclonal Antibody; Prostaglandin Dehydrogenase 1 (HPGD; PGDH1; 15-PGDH; hydroxyprostaglandin dehydrogenase 15-(NAD); Prostaglandin dehydrogenase 1); Anti -Prostaglandin Dehydrogenase 1 (HPGD; anti-Prostaglandin Dehydrogenase 1 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human 15-PGDH.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Applicable Applications for anti-Prostaglandin Dehydrogenase 1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml
Dilution: ELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml
Immunogen
Recombinant human 15-hydroxyprostaglandin dehydrogenase aa1-266; P15428 (E. coli).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of 15-PGDH. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/ml goat anti-h15-PGDH.)
SDS-Page
(Detection of 15-PGDH. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/ml goat anti-h15-PGDH.)
Related Product Information for anti-Prostaglandin Dehydrogenase 1 antibody
15-PGDH (15-hydroxyprostaglandin dehydrogenase NAD-linked; also HPGD) is a 28-29kD member of the short-chain non-metalloenzyme family of dehydrogenases. It is widely expressed, and antagonizes COX-2 by catalyzing the oxidation of 15-OH prostaglandin to metabolically inactive 15-keto metabolites. Human 15-PGDH is 266aa in length. A Tyr at position #151 serves as a proton acceptor, and a Ser at #138 binds substrate. 15-PGDH functions as a nondisulfide-linked homodimer. There is one alternate splice form that shows a 12aa substitution for aa167-266. Full-length human 15-PGDH shares 88% aa identity with mouse 15-PGDH.
Product Categories/Family for anti-Prostaglandin Dehydrogenase 1 antibody
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Product Notes
The Prostaglandin Dehydrogenase 1 (Catalog #AAA621755) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Prostaglandin Dehydrogenase 1 (HPGD, PGDH1, 15-PGDH, hydroxyprostaglandin dehydrogenase 15-(NAD), Prostaglandin dehydrogenase 1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Prostaglandin Dehydrogenase 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml. Researchers should empirically determine the suitability of the Prostaglandin Dehydrogenase 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prostaglandin Dehydrogenase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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