Immunohistochemistry (IHC)
(Immunochemical staining of mouse PRSS8 in mouse lung with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Mouse Prostasin/PRSS8 Polyclonal Antibody | anti-PRSS8 antibody
Anti-Prostasin/PRSS8 Antibody, Rabbit Polyclonal
Gene Names
Prss8; fr; CAP1; mCAP1; C79772; AI313909; 2410039E18Rik
Reactivity
Mouse
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
Prostasin/PRSS8; Polyclonal Antibody; Anti-Prostasin/PRSS8 Antibody; Rabbit Polyclonal; Prostasin/PRSS8 Antibody; Rabbit PAb; Antigen Affinity Purified; protease; serine; 8; Anti-2410039E18Rik Antibody; Anti-AI313909 Antibody; Anti-C79772 Antibody; Anti-CAP1 Antibody; Anti-fr Antibody; Anti-mCAP1 Antibody; anti-PRSS8 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse Prostasin/PRSS8
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PRSS8 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
IHC-P: 1:5000-1:20,000
ELISA: 1:25,000-1:50,000
IHC-P: 1:5000-1:20,000
Immunogen
Recombinant Mouse Prostasin/PRSS8 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Prostasin/PRSS8. Prostasin/PRSS8 specific IgG was purified by Mouse Prostasin/PRSS8 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of mouse PRSS8 in mouse lung with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse PRSS8 in mouse lung with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse PRSS8 in mouse pancreas with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse PRSS8 in mouse pancreas with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
Western Blot (WB)
(Anti-PRSS8 rabbit polyclonal antibody at 1:500 dilutionLane A: RAW264.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:37 kDaObserved band size:37 kDa)
Western Blot (WB)
(Anti-PRSS8 rabbit polyclonal antibody at 1:500 dilutionLane A: RAW264.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:37 kDaObserved band size:37 kDa)
Related Product Information for anti-PRSS8 antibody
Prostasin (Prss8), also known as channel activating protease 1 (CAP1), is a trypsinlike serine peptidase, and plays important roles in epithelial physiology. It is originally purified as an active, soluble enzyme from human seminal fluid and is highly expressed in prostate, lung, kidney, salivary gland and pancreas. Prostasin is expressed as a glycosyl-phosphatidylinositol (GPI)-anchored membrane protein in prostate epithelial cells, and also exists as a secreted proteolytic enzyme possibly via tryptic cleavage of its COOH-terminal hydrophobic domain. Prostasin is found to activate the epithelial sodium channel (ENaC) which is tightly regulated and is critical for maintaining salt and fluid balance in the lung and kidney in both normal and pathological conditions. Accordingly, prostasin has been proposed as a target for therapeutic inhibition in cystic fibrosis. Besides, prostasin inhibits prostate and breast cancer cell invasion in vitro, suggesting a functional role as a suppressor of tumor invasion, as well as a regulator of gene expression during inflammation.
Product Categories/Family for anti-PRSS8 antibody
References
Yu J.X., et al., (1995), Molecular cloning, tissue-specific expression, and cellular localization of human prostasin mRNA. J. Biol. Chem. 270:13483-13489.Ota T., et al., (2004), Complete sequencing and characterization of 21, 243 full-length human cDNAs.Nat. Genet. 36:40-45.Martin J., et al., (2004), The sequence and analysis of duplication-rich human chromosome 16.Nature 432:988-994.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
36,729 Da
NCBI Official Full Name
protease, serine, 8 (prostasin), isoform CRA_b, partial
NCBI Official Synonym Full Names
protease, serine 8 (prostasin)
NCBI Official Symbol
Prss8
NCBI Official Synonym Symbols
fr; CAP1; mCAP1; C79772; AI313909; 2410039E18Rik
NCBI Protein Information
prostasin
Protein Family
Research Articles on PRSS8
Similar Products
Product Notes
The PRSS8 (Catalog #AAA8111016) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Prostasin/PRSS8 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Prostasin/PRSS8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 IHC-P: 1:5000-1:20,000. Researchers should empirically determine the suitability of the PRSS8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prostasin/PRSS8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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