Sheep anti-Human Carbohydrate Sulfotransferase 1 Polyclonal Antibody | anti-CHST1 antibody

Carbohydrate Sulfotransferase 1 (CHST1, Carbohydrate (keratan sulfate Gal-6) sulfotransferase 1, C6ST, Carbohydrate Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1, GST-1, Keratan sulfate Gal-6 sulfotransferase, KS6ST, KSGal6ST, K

Cat. Num. AAA620110

• Gene Names: CHST1; C6ST; KS6ST; KSGal6ST
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Carbohydrate Sulfotransferase 1; Polyclonal Antibody; Carbohydrate Sulfotransferase 1 (CHST1; Carbohydrate (keratan sulfate Gal-6) sulfotransferase 1; C6ST; Carbohydrate Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; GST-1; Keratan sulfate Gal-6 sulfotransferase; KS6ST; KSGal6ST; K; Anti -Carbohydrate Sulfotransferase 1 (CHST1; anti-CHST1 antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CHST1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.

• Applicable Applications for anti-CHST1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 0.5-1.0ug/ml; Western Blot: 1.0ug/ml
• Immunogen: CHO cell-derived, recombinant human CHST1 aa24- 411.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(Detection of CHST1. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/ml sheep anti-hCHST1.)

Related Product Information for anti-CHST1 antibody

The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6 O or 4O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (2). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (3). CHST4, also

Product Categories/Family for anti-CHST1 antibody

Antibodies; Abs to Enzymes; Sulfotransferase

NCBI and Uniprot Product Information

• NCBI GI #: 4502841
• NCBI GeneID: 8534
• NCBI Accession #: NP_003645.1
• NCBI GenBank Nucleotide #: NM_003654.4
• UniProt Accession #: O43916; D3DQP2
• Molecular Weight: 46,715 Da
• NCBI Official Full Name: carbohydrate sulfotransferase 1
• NCBI Official Synonym Full Names: carbohydrate (keratan sulfate Gal-6) sulfotransferase 1
• NCBI Official Symbol: CHST1
• NCBI Official Synonym Symbols: C6ST; KS6ST; KSGal6ST
• NCBI Protein Information: carbohydrate sulfotransferase 1; KSST; GST-1; OTTHUMP00000233116; keratan sulfate Gal-6 sulfotransferase; carbohydrate (chondroitin 6/keratan) sulfotransferase 1; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1
• UniProt Protein Name: Carbohydrate sulfotransferase 1
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: CHST1
• UniProt Entry Name: CHST1_HUMAN

NCBI Description

This locus encodes a member of the keratin sulfotransferase family of proteins. The encoded enzyme catalyzes the sulfation of the proteoglycan keratin. [provided by RefSeq, Aug 2011]

Uniprot Description

CHST1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N- acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Protein type: EC 2.8.2.21; Glycan Metabolism - keratan sulfate biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: sulfotransferase activity; keratan sulfotransferase activity

Biological Process: keratan sulfate metabolic process; sulfur metabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; galactose metabolic process; pathogenesis; polysaccharide metabolic process; inflammatory response