SDS-Page
(Detection of Glyoxalase II with MBS621724. Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml goat anti-Glyoxalase II.)
Goat Glyoxalase II Polyclonal Antibody | anti-Mb0651c antibody
Glyoxalase II (GLO2, Hydroxyacylglutathione Hydrolase 2, Glx II)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Glyoxalase II; Polyclonal Antibody; Glyoxalase II (GLO2; Hydroxyacylglutathione Hydrolase 2; Glx II); Anti -Glyoxalase II (GLO2; anti-Mb0651c antibody
Host
Goat
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Glyoxalase II. Species Crossreactivity: mouse, rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-Mb0651c antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Immunogen
E. coli-derived recombinant human Glyoxalase II (aa49-254; Accession # Q16775).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of Glyoxalase II with MBS621724. Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml goat anti-Glyoxalase II.)
SDS-Page
(Detection of Glyoxalase II with MBS621724. Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml goat anti-Glyoxalase II.)
Related Product Information for anti-Mb0651c antibody
Glyoxalase II (GLO2; also hydroxyacylglutathione hydrolase 2 and Glx II) is a monomeric, cytosolic 29kD member of the Glyoxylase II family, metallo-B-lactamase superfamily of enzymes. It is expressed in liver and kidney, and converts GLO1-generated lactoylglutathione into lactate and GSH. It is upregulated by p63 and p73, and thus serves as a p53-associated survival factor. Human GLO2 is 260aa in length. It binds two atoms of Zn and contains one substrate binding region (aa143-145 plus 249-252). The 29kD form represents 80-90% of cellular GLO2. There is also a 32-33kD, 308aa mitochondrial isoform that arises via the use of an alternative start site. Its function is unclear, given that mitochondria do not contain GLO1. There is an additional potential isoform that shows an insertion of nine aa after Thr167. Human GLO2 shares approximately 90% aa identity with mouse GLO2.
Product Categories/Family for anti-Mb0651c antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
glyoxalase II
NCBI Official Symbol
Mb0651c
NCBI Protein Information
glyoxalase II
Similar Products
Product Notes
The Mb0651c (Catalog #AAA621724) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Glyoxalase II (GLO2, Hydroxyacylglutathione Hydrolase 2, Glx II) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glyoxalase II can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 0.5-1ug/ml Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the Mb0651c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glyoxalase II, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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