Goat anti-Human Microphthalmia Polyclonal Antibody | anti-Mitf antibody

Microphthalmia (Transcription Factor, MITF)

Cat. Num. AAA620052

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Microphthalmia; Polyclonal Antibody; Microphthalmia (Transcription Factor; MITF); Anti -Microphthalmia (Transcription Factor; anti-Mitf antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MITF. Species sequence homology: mouse (96%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol.

• Applicable Applications for anti-Mitf antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Immunohistochemistry, and Western Blot.; Dilution: Western Blot: 1ug/ml; ELISA: 0.5-1ug/ml; Immunohistochemistry: 3-10ug/ml
• Immunogen: E. coli-derived, recombinant human MITF (aa119-289).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(Detection of MITF with MBS620052. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml goat anti-hMITF.)

Related Product Information for anti-Mitf antibody

MITF (Microphthalmia-associated transcription factor) is a member of the MiT/TFE family of molecules. Although is has a predicted MW of 58kD, it runs anomalously in SDS-PAGE at 57-66kD. MITF-A1 is found in melanocytes and RPEs where it regulates melanin synthesis via tyrosinase and TRP-1 gene expression. Human MITF-A1 is 526aa in length. It contains a bHLH DNA binding region (aa309-369) and a Leu-zipper domain (aa374-395). There are three phosphorylation sites at Ser54/405/414, and two SUMOylation sites at Arg289/423. MITF-A1 acts as either a homodimer, or heterodimer with TFE3, TFEB or TFEC. Multiple splice variants exist, with each variant expressing one of two isoforms that are defined by the presence (#1), or absence (#2), of aa294-299. One variant has a deletion of aa139-194, a second has an 11aa substitution for aa1-118, and three others show variable substitutions over aa1-35. Over aa119-289, human MITF-A1 shares 96% aa identity with mouse MITF-A1.

Product Categories/Family for anti-Mitf antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 3319231
• NCBI GeneID: 25094
• UniProt Accession #: O88368
• Molecular Weight: 13,155 Da
• NCBI Official Full Name: microphthalmia associated transcription factor
• NCBI Official Synonym Full Names: microphthalmia-associated transcription factor
• NCBI Official Symbol: Mitf
• NCBI Protein Information: microphthalmia-associated transcription factor
• UniProt Protein Name: Microphthalmia-associated transcription factor
• Protein Family: Microphthalmia-associated transcription factor
• UniProt Gene Name: Mitf
• UniProt Entry Name: MITF_RAT

NCBI Description

basic helix-loop-helix-leucine zipper transcription factor; may play a role in osteoclast function [RGD, Feb 2006]

Uniprot Description

MITF: a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. Plays a critical role in the differentiation of various cell types including neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Two isoforms are known: the M-isoform is expressed exclusively in melanocytes, while the A-isoform has a much broader range of expression. Mutations in MITF can lead to Waardenburg syndrome. Ten alternatively spliced isoforms have been described.

Protein type: DNA-binding; Oncoprotein; Transcription factor

Cellular Component: protein complex; nucleus

Molecular Function: protein dimerization activity; RNA polymerase II transcription factor activity, enhancer binding; DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; Wnt receptor signaling pathway; cell fate commitment; camera-type eye development; positive regulation of transcription, DNA-dependent; osteoclast differentiation; negative regulation of transcription from RNA polymerase II promoter; bone remodeling; regulation of cell proliferation; regulation of osteoclast differentiation; regulation of transcription, DNA-dependent; pigmentation; regulation of gene expression; melanocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; protein complex assembly; cell differentiation; negative regulation of apoptosis