SDS-Page
(Detection of PEA-15. Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/ml sheep anti-PEA-15.)
Sheep anti-Human, Mouse PEA-15 Polyclonal Antibody | anti-PEA15 antibody
PEA-15 (phosphoprotein enriched in astrocytes 15 Astrocytic phosphoprotein PEA-15, HMAT1, HUMMAT1H, MAT1, MAT1H, PED, Phosphoprotein enriched in diabetes)
Gene Names
PEA15; PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H; PEA15
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
PEA-15; Polyclonal Antibody; PEA-15 (phosphoprotein enriched in astrocytes 15 Astrocytic phosphoprotein PEA-15; HMAT1; HUMMAT1H; MAT1; MAT1H; PED; Phosphoprotein enriched in diabetes); Anti -PEA-15 (phosphoprotein enriched in astrocytes 15 Astrocytic phosphoprotein PEA-15; anti-PEA15 antibody
Host
Sheep
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human and mouse PEA-15.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS. 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Applicable Applications for anti-PEA15 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Dilution: ELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml
Western Blot: 1.0ug/ml
Immunogen
Recombinant human PEA-15 aa 2-130; Q15121 (E. Coli).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of PEA-15. Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/ml sheep anti-PEA-15.)
SDS-Page
(Detection of PEA-15. Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/ml sheep anti-PEA-15.)
Related Product Information for anti-PEA15 antibody
PEA-15 (Phosphoprotein enriched in astrocytes of 15kD) is a 15kD cytoplasmic protein that regulates cell proliferation and apoptosis. It is expressed in multiple cell types such as astrocytes; skeletal muscle and adipocytes. Human PEA-15 is 130aa in length. It contains a death effector domain (aa3-81), plus two regulatory Ser phosphorylation sites. In a quiescent cell, it is either non-phosphorylated or constitutively phosphorylated at Ser116. Non-phosphorylated PEA-15 binds ERK, inhibiting cell proliferation. Phosphorylation at Ser116 promotes PEA-15 binding to
Product Categories/Family for anti-PEA15 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
15,040 Da
NCBI Official Full Name
PEA-15
NCBI Official Synonym Full Names
phosphoprotein enriched in astrocytes 15
NCBI Official Symbol
PEA15
NCBI Official Synonym Symbols
PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H; PEA15
NCBI Protein Information
astrocytic phosphoprotein PEA-15; OTTHUMP00000029887; homolog of mouse MAT-1 oncogene; phosphoprotein enriched in diabetes; Phosphoprotein enriched in astrocytes, 15kD; 15 kDa phosphoprotein enriched in astrocytes
UniProt Protein Name
Astrocytic phosphoprotein PEA-15
Protein Family
UniProt Gene Name
PEA15
UniProt Entry Name
PEA15_HUMAN
NCBI Description
PEA15 is a death effector domain (DED)-containing protein predominantly expressed in the central nervous system, particularly in astrocytes.[supplied by OMIM]
Uniprot Description
PEA-15: an ubiquitously expressed anti-apoptotic protein. Inhibits both Fas- and Tnfrsf1a/Tnfr1-mediated caspase-8 activity and apoptosis. Interacts with CASP8/FLICE and FADD. Regulates glucose transport by controlling both the content of GLUT1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of GLUT4 from the cell interior to the surface. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.
Protein type: Motility/polarity/chemotaxis; Apoptosis
Chromosomal Location of Human Ortholog: 1q21.1
Cellular Component: microtubule associated complex; cytoplasm
Molecular Function: protein binding
Biological Process: negative regulation of glucose import; transport; apoptosis; DNA damage checkpoint; carbohydrate transport; response to morphine
Research Articles on PEA15
1. Observational study of gene-disease association. (HuGE Navigator)
2. Results suggest that PEA-15 expression is likely to be associated with the tumorigenesis of alignant pleural mesothelioma.
3. Strategies are being devised to target key signaling events in PED/PEA-15 action aimed at improving glucose tolerance and at facilitating cancer cell death.
4. transfection with PED siRNA, but not with cFLIP siRNA, sensitized NSCLC cells to TRAIL-induced cell death
5. PTEN influences Fas signaling, at least in part, by regulating PEA-15 phosphorylation and activity that, in turn, regulate the ability of Bcl-2 to suppress Fas-induced apoptosis.
6. COUP-TFII antagonizes the repression of the PED/PEA-15 gene by HNF-4alpha.
7. PEA-15 expression is an important prognostic marker in ovarian cancer.
8. Elevated PEA-15 levels provide a survival advantage for glioblastoma cells confronted with a poor microenvironment and low glucose levels.
9. PEA-15 functions as a scaffold to enhance extracellular signal-regulated MAP kinase (ERK) activation of ribosomal S6 kinase polypeptide RSK2, an activity that is regulated by phosphorylation.
10. In addition to sequestering protein kinases ERK1/2 in the cytoplasm, PEA-15 has the potential to modulate the activity of ERK2 in cells by competing directly with proteins that contain D-recruitment sites.
2. Results suggest that PEA-15 expression is likely to be associated with the tumorigenesis of alignant pleural mesothelioma.
3. Strategies are being devised to target key signaling events in PED/PEA-15 action aimed at improving glucose tolerance and at facilitating cancer cell death.
4. transfection with PED siRNA, but not with cFLIP siRNA, sensitized NSCLC cells to TRAIL-induced cell death
5. PTEN influences Fas signaling, at least in part, by regulating PEA-15 phosphorylation and activity that, in turn, regulate the ability of Bcl-2 to suppress Fas-induced apoptosis.
6. COUP-TFII antagonizes the repression of the PED/PEA-15 gene by HNF-4alpha.
7. PEA-15 expression is an important prognostic marker in ovarian cancer.
8. Elevated PEA-15 levels provide a survival advantage for glioblastoma cells confronted with a poor microenvironment and low glucose levels.
9. PEA-15 functions as a scaffold to enhance extracellular signal-regulated MAP kinase (ERK) activation of ribosomal S6 kinase polypeptide RSK2, an activity that is regulated by phosphorylation.
10. In addition to sequestering protein kinases ERK1/2 in the cytoplasm, PEA-15 has the potential to modulate the activity of ERK2 in cells by competing directly with proteins that contain D-recruitment sites.
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Product Notes
The PEA15 pea15 (Catalog #AAA622921) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The PEA-15 (phosphoprotein enriched in astrocytes 15 Astrocytic phosphoprotein PEA-15, HMAT1, HUMMAT1H, MAT1, MAT1H, PED, Phosphoprotein enriched in diabetes) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PEA-15 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml. Researchers should empirically determine the suitability of the PEA15 pea15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PEA-15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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