Goat anti-Human, Rat Neuroligin 2 Polyclonal Antibody | anti-NLGN2 antibody

Neuroligin 2 (NLGN2, KIAA1366)

Cat. Num. AAA622847

• Gene Names: NLGN2; KIAA1366
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Neuroligin 2; Polyclonal Antibody; Neuroligin 2 (NLGN2; KIAA1366); Anti -Neuroligin 2 (NLGN2; anti-NLGN2 antibody

• Host: Goat
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human and rat Neuroligin 2 extracellular domain.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS,5% trehalose. Reconstitute with 40-50% glycerol, PBS.

• Applicable Applications for anti-NLGN2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Dilution: ELISA: 0.5-1.0ug/ml; Western Blot: 1.0ug/ml
• Immunogen: NS0-derived rhNeuroligin 2 extracellular domain aa15-660; EAW90197.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(Detection of Neuroligin 2. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with N2160-02B (1.0ug/ml).)

Related Product Information for anti-NLGN2 antibody

NLGN2 (Neuroligin 2) is a 105kD member of the type-B carboxylesterase family of enzymes. It is expressed at GABAergic synapses, and is assumed to play both an organizing and regulatory role in inhibitory synapse signaling. It is suggested to bind to beta -neurexin-1, 2 and 3 as a dimer in trans, and to interact cytoplasmically with PDZ-domain containing proteins. Mature human NLGN2 is an 821aa type I transmembrane (TM) glycoprotein. It contains a nonfunctional type-B lipase domain (aa40-601), a TM segment (aa678-698), and a cytoplasmic tail with a PZD-binding region (aa785-835). There are potential splice forms. Individually, or in combination, there is an alternate start site at Met512, a deletion of aa153-169, and a 99aa substitution for aa 806-835. Over aa1-677, human NLGN2 shares 98% aa identity with mouse NLGN2.

Product Categories/Family for anti-NLGN2 antibody

Antibodies; Abs to Neuroligins

NCBI and Uniprot Product Information

• NCBI GI #: 21309947
• NCBI GeneID: 57555
• UniProt Accession #: Q8NFZ4; Q9P2I1
• Molecular Weight: 90,820 Da
• NCBI Official Full Name: neuroligin 2
• NCBI Official Synonym Full Names: neuroligin 2
• NCBI Official Symbol: NLGN2
• NCBI Official Synonym Symbols: KIAA1366
• NCBI Protein Information: neuroligin-2; OTTHUMP00000135277
• UniProt Protein Name: Neuroligin-2
• Protein Family: Neuroligin
• UniProt Gene Name: NLGN2
• UniProt Synonym Gene Names: KIAA1366
• UniProt Entry Name: NLGN2_HUMAN

NCBI Description

This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. [provided by RefSeq]

Uniprot Description

NLGN2: Transmembrane scaffolding protein involved in cell-cell interactions via its interactions with neurexin family members. Mediates cell-cell interactions both in neurons and in other types of cells, such as Langerhans beta cells. Plays a role in synapse function and synaptic signal transmission, especially via gamma- aminobutyric acid receptors (GABA(A) receptors). Functions by recruiting and clustering synaptic proteins. Promotes clustering of postsynaptic GABRG2 and GPHN. Modulates signaling by inhibitory synapses, and thereby plays a role in controlling the ratio of signaling by excitatory and inhibitory synapses and information processing. Required for normal signal amplitude from inhibitory synapses, but is not essential for normal signal frequency. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. Mediates cell-cell interactions between Langerhans beta cells and modulates insulin secretion. Belongs to the type-B carboxylesterase/lipase family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: presynaptic membrane; extracellular space; postsynaptic membrane; cell surface; membrane; integral to plasma membrane; synapse; cell junction

Molecular Function: neurexin binding; receptor activity; cell adhesion molecule binding

Biological Process: regulation of synaptic transmission; metabolic process; positive regulation of synaptic transmission, GABAergic; neurological control of breathing; sensory perception of pain; positive regulation of synaptic transmission, glutamatergic; positive regulation of synaptogenesis; cell-cell adhesion; synaptogenesis; synapse organization and biogenesis; neuron adhesion; neuromuscular process controlling balance; intercellular junction maintenance