Sheep anti-Mouse Mrc2 Polyclonal Antibody | anti-MRC2 antibody

Mrc2 (Macrophage Mannose Receptor 2, uPARAP, Endo180)

Cat. Num. AAA615474

• Gene Names: MRC2; CD280; UPARAP; CLEC13E; ENDO180; FLJ35911; KIAA0709
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Mrc2; Polyclonal Antibody; Mrc2 (Macrophage Mannose Receptor 2; uPARAP; Endo180); Anti -Mrc2 (Macrophage Mannose Receptor 2; anti-MRC2 antibody

• Host: Sheep
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Mrc2.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.

• Applicable Applications for anti-MRC2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Direct ELISA and Western Blot.; Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rmMrc2 is ~ 1ng/well.; Western Blot: 1ug/ml
• Immunogen: NS0-derived rmMrc2 extracellular domain. (aa 31 - 1413)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(Detection of Mrc2 with AF4789. Mouse tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0 ug/mL MBS615474.)

Related Product Information for anti-MRC2 antibody

Mrc2 (macrophage mannose receptor 2; also uPARAP/Endo180) is a 180kD member of the mannose receptor family. It is found on fibroblasts, macrophages and endothelial cells. Mrc2 binds and internalizes glycoproteins plus collagen types I and IV. Mouse Mrc2 is a type I transmembrane glycoprotein that is 1479 amino acids (aa) in length. It possesses a 1383 aa extracellular domain (ECD) (aa 31-1479) and a short 45 aa cytoplasmic tail. The ECD contains eight C-type lectin domains plus one fibronectin type II domain (aa 181-229) that binds collagen. There are two splice variants, one which shows an eight aa substitution for aa 173-1479, and a second that shows a 72 aa substitution for aa 982-1479. Over aa 31-1413, mouse Mrc2 shares 91% and 97% aa identity with human and rat Mrc2, respectively.

Product Categories/Family for anti-MRC2 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 119614748
• NCBI GeneID: 9902
• UniProt Accession #: Q9UBG0; Q7LGE7; Q9Y5P9; A6H8K4; D3DU08
• Molecular Weight: 166,674 Da
• NCBI Official Full Name: mannose receptor, C type 2, isoform CRA_a
• NCBI Official Synonym Full Names: mannose receptor, C type 2
• NCBI Official Symbol: MRC2
• NCBI Official Synonym Symbols: CD280; UPARAP; CLEC13E; ENDO180; FLJ35911; KIAA0709
• NCBI Protein Information: C-type mannose receptor 2; endocytic receptor 180; UPAR-associated protein; macrophage mannose receptor 2; urokinase receptor-associated protein; C-type lectin domain family 13 member E; endocytic receptor (macrophage mannose receptor family); urokinase plasminogen activator receptor-associated protein; urokinase-type plasminogen activator receptor-associated protein
• UniProt Protein Name: C-type mannose receptor 2
• Protein Family: C-type mannose receptor
• UniProt Gene Name: MRC2
• UniProt Synonym Gene Names: CLEC13E; ENDO180; KIAA0709; UPARAP
• UniProt Entry Name: MRC2_HUMAN

NCBI Description

MRC2 is a recycling endocytic receptor that functions in cell motility and remodeling of the extracellular matrix by promoting cell migration and uptake of collagens for intracellular degradation (Wienke et al., 2007 [PubMed 17974964]).[supplied by OMIM]

Uniprot Description

MRC2: May play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix co-operating with the matrix metalloproteinases (MMPs).

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: focal adhesion; membrane; integral to membrane

Molecular Function: collagen binding; protein binding; carbohydrate binding

Biological Process: osteoblast differentiation; collagen catabolic process; endocytosis