Dilution Information
Rabbit anti-Human MGMT/AGAT Monoclonal Antibody | anti-MGMT/AGAT antibody
MGMT/AGAT (EP337)
Reactivity
Human
Synonyms
MGMT/AGAT; Monoclonal Antibody; MGMT/AGAT (EP337); mgmt; agat; anti-mgmt; anti-agat; agt; anti-agt; anti-MGMT/AGAT antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
EP337
Form/Format
Paraffin, Frozen
MGMT is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH7.5, containing BSA and sodium azide as a preservative.
MGMT is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH7.5, containing BSA and sodium azide as a preservative.
Immunogen
A synthetic peptide corresponding to residues of the human MGMT protein.
Control
Breast, Cervix, Fallopian Tube, Prostate, Testis, Transitional Cell Carcinoma, Ductal Breast Carcinoma, Endometrial Carcinoma
Localization
Cytoplasmic, Nuclear
Preparation and Storage
Store at 2-8 degree C (Control Slides: Store at 20-25 degree C)
Dilution Information
Dilution Information
Immunohistochemistry (IHC)
(MGMT Antibody Immunohistochemistry on an FFPE Lung Adenocarcinoma Tissue)
Immunohistochemistry (IHC)
(MGMT Antibody Immunohistochemistry on an FFPE Lung Adenocarcinoma Tissue)
Related Product Information for anti-MGMT/AGAT antibody
MGMT is a house-keeping gene expressed in all tissues, and its promoter methylation and resulting down-regulation of the AGT protein varies among tumor types. The MGMT promoter contains many regulatory domains, and methylation can help decrease protein expression and therefore resistance to drugs, improving effectiveness of alkylating treatments.
High MGMT antibody expression has been reported in Glioma, Myeloma, Melanoma, Colon and Pancreatic Cancers, where inhibition or down-regulation make alkylating drug treatments more effective. MGMT expression is lost in some cancer types such as Lymphomas, Non-Small Cell Lung Cancer, Astrocyte Tumors, Breast, and Prostate Cancer, where it may fail to prevent mutations. A study detected protein expression of MGMT by IHC and MGMT promoter methylation detected by methylation-specific polymerase chain reaction (MSP) were performed in a series of newly identified Glioblastomas and found that the MGMT status detected by either IHC or MSP was significantly correlated with the treatment response and survival of Glioblastoma patients. MGMT methylation is frequently observed in sporadic colorectal cancer and was recently correlated with the C>T allele at SNP rs16906252, within the transcriptional enhancer element of the promoter. MGMT methylation has also been associated with KRAS mutations, particularly G>A transitions. Another study address the association between sequence variants within the MGMT (O(6)-methylguanine-DNA methyltransferase) promoter-enhancer region and methylation of MGMT in premalignant lesions from smokers and lung adenocarcinomas, their biological effects on gene regulation, and targeting MGMT for therapy and found strong evidence that the A allele of a MGMT promoter-enhancer SNP is a key determinant for MGMT methylation in lung carcinogenesis. Moreover, TMZ treatment may benefit a subset of lung cancer patients methylated for MGMT.
High MGMT antibody expression has been reported in Glioma, Myeloma, Melanoma, Colon and Pancreatic Cancers, where inhibition or down-regulation make alkylating drug treatments more effective. MGMT expression is lost in some cancer types such as Lymphomas, Non-Small Cell Lung Cancer, Astrocyte Tumors, Breast, and Prostate Cancer, where it may fail to prevent mutations. A study detected protein expression of MGMT by IHC and MGMT promoter methylation detected by methylation-specific polymerase chain reaction (MSP) were performed in a series of newly identified Glioblastomas and found that the MGMT status detected by either IHC or MSP was significantly correlated with the treatment response and survival of Glioblastoma patients. MGMT methylation is frequently observed in sporadic colorectal cancer and was recently correlated with the C>T allele at SNP rs16906252, within the transcriptional enhancer element of the promoter. MGMT methylation has also been associated with KRAS mutations, particularly G>A transitions. Another study address the association between sequence variants within the MGMT (O(6)-methylguanine-DNA methyltransferase) promoter-enhancer region and methylation of MGMT in premalignant lesions from smokers and lung adenocarcinomas, their biological effects on gene regulation, and targeting MGMT for therapy and found strong evidence that the A allele of a MGMT promoter-enhancer SNP is a key determinant for MGMT methylation in lung carcinogenesis. Moreover, TMZ treatment may benefit a subset of lung cancer patients methylated for MGMT.
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Product Notes
The MGMT/AGAT (Catalog #AAA370503) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MGMT/AGAT (EP337) reacts with Human and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "MGMT/AGAT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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