SDS-Page
(JHDM1D-l protein gel. JHDM1D-l (10 ?g) run on a 4-20% SDS-PAGE gel and stained with Coomassie blue.)
JHDM1D-l recombinant protein
Recombinant JHDM1D-l protein
Gene Names
KDM7A; JHDM1D
Synonyms
JHDM1D-l; Recombinant JHDM1D-l protein; KDM7A; Lysine Demethylase 7A; Jumonji C Domain Containing Histone Demethylase 1 Homolog D; JHDM1D-l recombinant protein
Host
E Coli
Form/Format
20ug of recombinant JHDM1D-l protein expressed in E Coli cells and supplied in 20mM Bis-Tris, pH5.5, 200mM NaCl, 5% (v/v) glycerol and 1mM DTT.
Protein Species
Human
Tag
GST-Tag
Notes
Recombinant JHDM1D-l is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(JHDM1D-l protein gel. JHDM1D-l (10 ?g) run on a 4-20% SDS-PAGE gel and stained with Coomassie blue.)
SDS-Page
(JHDM1D-l protein gel. JHDM1D-l (10 ?g) run on a 4-20% SDS-PAGE gel and stained with Coomassie blue.)
Testing Data
(JHDM1D-l activity assay. Recombinant JHDM1D-l activity measured using a fluorescence-based demethylation assay.)
Testing Data
(JHDM1D-l activity assay. Recombinant JHDM1D-l activity measured using a fluorescence-based demethylation assay.)
Related Product Information for JHDM1D-l recombinant protein
Short Description: Recombinant JHDM1D-l, that includes amino acids 1-488 of JHDM1D (accession number NP_085150) was generated by expressing a GST fusion protein containing residues 1-488 of JHDM1D in E Coli, followed by affinity purification and cleavage of the GST-Tag with thrombin to produce a protein with an observed molecular weight of 54.990 kDa. JHDM1D (Jumonji C Domain Containing Histone Demethylase 1 Homolog D), also known as KIAA1718 and KDM7, is a member of the JmjC-containing (Jumonji-C) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JHDM1D harbors two N-terminal domains, a PHD finger that binds trimethylated lysine 4 of histone H3 (H3K4me3) and a Jumonji domain that demethylates Histone H3 dimethyl Lys9 (H3K9me2), Histone H3 dimethyl Lys27 (H3K27me2)(which are all modifications associated with transcriptional repression), Histone H3 dimethyl Lys36 (H3K36me2) and also Histone H4 monomethyl Lys20 (H4K20me1) via an oxidative pathway that requires the presence of Fe(II) and a-ketoglutarate as cofactors. In the presence of H3K4me3, JHDM1D has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. JHDM1D demethylates H3K9me2 in the absence of H3K4me3. JHDM1D has activity toward H4K20me1 only when a nucleosome is used as a substrate, not when a histone octamer is used as a substrate. JHDM1D enzyme is required for brain development. JHDM1D-l is a truncated protein (aa 1-488) that includes the PHD and the catalytic Jumonji domain of JHDM1D. The protein contains different segments for both recognizing and removing opposing methyl groups. While the PHD binds H3K4me3, an alteration associated with transcriptional activation, the Jumonji (catalytic) domain demethylates H3K27me2, a mechanism associated with transcriptional repression. The enzymes' catalytic activity and substrate specificity is thereby enhanced through the simultaneous binding of multiple domains. The ability to apprehend the function of individual methyl marks could lead to a marked understanding of the language of the histone code. Recombinant JHDM1D-l can be used for enzyme kinetics and inhibitor screening studies.
Background: JHDM1D (Jumonji C Domain Containing Histone Demethylase 1 Homolog D), also known as KIAA1718 and KDM7, is a member of the JmjC-containing (Jumonji-C) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JHDM1D harbors two N-terminal domains, a PHD finger that binds trimethylated lysine 4 of histone H3 (H3K4me3) and a Jumonji domain that demethylates Histone H3 dimethyl Lys9 (H3K9me2), Histone H3 dimethyl Lys27 (H3K27me2)(which are all modifications associated with transcriptional repression), Histone H3 dimethyl Lys36 (H3K36me2) and also Histone H4 monomethyl Lys20 (H4K20me1) via an oxidative pathway that requires the presence of Fe(II) and a-ketoglutarate as cofactors. In the presence of H3K4me3, JHDM1D has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. JHDM1D demethylates H3K9me2 in the absence of H3K4me3. JHDM1D has activity toward H4K20me1 only when a nucleosome is used as a substrate, not when a histone octamer is used as a substrate. JHDM1D enzyme is required for brain development. JHDM1D-l is a truncated protein (aa 1-488) that includes the PHD and the catalytic Jumonji domain of JHDM1D. The protein contains different segments for both recognizing and removing opposing methyl groups. While the PHD binds H3K4me3, an alteration associated with transcriptional activation, the Jumonji (catalytic) domain demethylates H3K27me2, a mechanism associated with transcriptional repression. The enzymes' catalytic activity and substrate specificity is thereby enhanced through the simultaneous binding of multiple domains. The ability to apprehend the function of individual methyl marks could lead to a marked understanding of the language of the histone code.
Background: JHDM1D (Jumonji C Domain Containing Histone Demethylase 1 Homolog D), also known as KIAA1718 and KDM7, is a member of the JmjC-containing (Jumonji-C) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JHDM1D harbors two N-terminal domains, a PHD finger that binds trimethylated lysine 4 of histone H3 (H3K4me3) and a Jumonji domain that demethylates Histone H3 dimethyl Lys9 (H3K9me2), Histone H3 dimethyl Lys27 (H3K27me2)(which are all modifications associated with transcriptional repression), Histone H3 dimethyl Lys36 (H3K36me2) and also Histone H4 monomethyl Lys20 (H4K20me1) via an oxidative pathway that requires the presence of Fe(II) and a-ketoglutarate as cofactors. In the presence of H3K4me3, JHDM1D has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. JHDM1D demethylates H3K9me2 in the absence of H3K4me3. JHDM1D has activity toward H4K20me1 only when a nucleosome is used as a substrate, not when a histone octamer is used as a substrate. JHDM1D enzyme is required for brain development. JHDM1D-l is a truncated protein (aa 1-488) that includes the PHD and the catalytic Jumonji domain of JHDM1D. The protein contains different segments for both recognizing and removing opposing methyl groups. While the PHD binds H3K4me3, an alteration associated with transcriptional activation, the Jumonji (catalytic) domain demethylates H3K27me2, a mechanism associated with transcriptional repression. The enzymes' catalytic activity and substrate specificity is thereby enhanced through the simultaneous binding of multiple domains. The ability to apprehend the function of individual methyl marks could lead to a marked understanding of the language of the histone code.
Product Categories/Family for JHDM1D-l recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
106kDa
NCBI Official Full Name
lysine-specific demethylase 7A
NCBI Official Synonym Full Names
lysine demethylase 7A
NCBI Official Symbol
KDM7A
NCBI Official Synonym Symbols
JHDM1D
NCBI Protein Information
lysine-specific demethylase 7A
UniProt Protein Name
Lysine-specific demethylase 7
UniProt Gene Name
JHDM1D
UniProt Synonym Gene Names
KDM7; KIAA1718
UniProt Entry Name
KDM7_HUMAN
Uniprot Description
JHDM1D: Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate. Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.11.-
Chromosomal Location of Human Ortholog: 7q34
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: zinc ion binding; histone demethylase activity (H3-K36 specific); iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; histone demethylase activity (H3-K9 specific); methylated histone residue binding
Biological Process: establishment and/or maintenance of chromatin architecture; histone H3-K9 demethylation; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; midbrain development
Research Articles on JHDM1D-l
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Product Notes
The JHDM1D-l jhdm1d (Catalog #AAA388175) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "JHDM1D-l, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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