SDS-Page
(Recombinant KDM2A / FBXL11 protein gel. KDM2A / FBXL11 protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue. MW: 128 kDa Purity: > 85%)
KDM2A/FBXL11 recombinant protein
Recombinant KDM2A/FBXL11 protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant KDM2A / FBXL11 protein gel. KDM2A / FBXL11 protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue. MW: 128 kDa Purity: > 85%)
SDS-Page
(Recombinant KDM2A / FBXL11 protein gel. KDM2A / FBXL11 protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue. MW: 128 kDa Purity: > 85%)
Testing Data
(MALDI-TOF for KDM2A / FBXL11 protein activity 3 ?M H3K36me2 (21-44 aa) peptide was incubated with 0, 100 nM of KDM2A / FBXL11 protein in reaction buffer containing 50 mM HEPES-NaOH pH 7.5, 100 ?M 2-oxoglutarate, 100 ?M ascorbate, 50 ?M (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP for 2 hr at room temperature. Single H3K36me2 peptide was used as negative control. MALDI-TOF was used for detection.)
Testing Data
(MALDI-TOF for KDM2A / FBXL11 protein activity 3 ?M H3K36me2 (21-44 aa) peptide was incubated with 0, 100 nM of KDM2A / FBXL11 protein in reaction buffer containing 50 mM HEPES-NaOH pH 7.5, 100 ?M 2-oxoglutarate, 100 ?M ascorbate, 50 ?M (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP for 2 hr at room temperature. Single H3K36me2 peptide was used as negative control. MALDI-TOF was used for detection.)
Background: Lysine (K)-specific demethylase 2A (KDM2A), also known as FBXL11, JHDM1A, CXXC8, nucleates at CpG islands and specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
Uniprot Description
FBXL11: Histone demethylase that specifically demethylates 'Lys- 36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys- 36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. Belongs to the JHDM1 histone demethylase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Oxidoreductase; Demethylase; EC 1.14.11.27
Chromosomal Location of Human Ortholog: 11q13.2
Cellular Component: nucleoplasm; nuclear chromatin; nucleolus; nucleus
Molecular Function: unmethylated CpG binding; zinc ion binding; histone demethylase activity (H3-K36 specific)
Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; double-strand break repair via nonhomologous end joining
Research Articles on KDM2A/FBXL11
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Product Notes
The KDM2A/FBXL11 kdm2a (Catalog #AAA388196) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KDM2A/FBXL11, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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