SDS-Page
(Recombinant JMJD1A / KDM3A protein gel. JMJD1A / KDM3A protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue.)
JMJD1A/KDM3A recombinant protein
Recombinant JMJD1A/KDM3A protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant JMJD1A / KDM3A protein gel. JMJD1A / KDM3A protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue.)
SDS-Page
(Recombinant JMJD1A / KDM3A protein gel. JMJD1A / KDM3A protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue.)
Testing Data
(HTRF assay for JMJD1A / KDM3A activity 3 uM H3K9me1 peptide was incubated with JMJD1A / KDM3A in reaction buffer for 2 hours at room temperature. Anti-H3K9me0 antibody was used to detect reaction product.)
Testing Data
(HTRF assay for JMJD1A / KDM3A activity 3 uM H3K9me1 peptide was incubated with JMJD1A / KDM3A in reaction buffer for 2 hours at room temperature. Anti-H3K9me0 antibody was used to detect reaction product.)
Testing Data
(HTRF assay for JMJD1A / KDM3A activity 3 uM H3K9me1 peptide was incubated with JMJD1A / KDM3A in reaction buffer for 2 hours at room temperature. Anti-H3K9me0 antibody was used to detect reaction product.)
Testing Data
(HTRF assay for JMJD1A / KDM3A activity 3 uM H3K9me1 peptide was incubated with JMJD1A / KDM3A in reaction buffer for 2 hours at room temperature. Anti-H3K9me0 antibody was used to detect reaction product.)
Background: KDM3A (lysine (K)-specific demethylase 3A), also known as JMJD1A (Jumonji Domain Containing 1A) is a histone demethylase that preferentially demethylates mono- and dimethylated lysine 9 of histone H3, with a preference for the dimethylated residue. KDM3A has little or no activity on trimethylated lysine 9. KDM3A is involved in hormone-dependent transcriptional activation by participating in the recruitment to androgen-receptor target genes resulting in H3 lysine 9 demethylation and transcriptional activation. KDM3A is also involved in spermatogenesis where it regulates expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. KDM3A contributes to obesity resistance through its regulation of metabolic genes such as PPARa and UCP1.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Uniprot Description
JMJD1A: Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. Belongs to the JHDM2 histone demethylase family.
Protein type: EC 1.14.11.-; Demethylase; Oxidoreductase; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 2p11.2
Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus
Molecular Function: androgen receptor binding; dioxygenase activity; iron ion binding; transcription factor activity
Biological Process: negative regulation of histone H3-K9 methylation; formaldehyde biosynthetic process; establishment and/or maintenance of chromatin architecture; spermatid nuclear elongation; histone H3-K9 demethylation; hormone-mediated signaling; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter
Research Articles on JMJD1A/KDM3A
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Product Notes
The JMJD1A/KDM3A kdm3a (Catalog #AAA388170) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "JMJD1A/KDM3A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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