SDS-Page
(Recombinant LSD1 / KDM1A protein gel. LSD1 / KDM1A protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue. MW: 110 kDa Purity: > 91%)
LSD1/KDM1A recombinant protein
Recombinant LSD1/KDM1A protein
Purity
The recombinant protein is >90% pure by SDS-PAGE.
Synonyms
LSD1/KDM1A; Recombinant LSD1/KDM1A protein; Lysine Demethylase 1A; Flavin-Containing Amine Oxidase Domain-Containing Protein 2; Lysine-Specific Histone Demethylase 1; LSD1/KDM1A recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >90% pure by SDS-PAGE.
Form/Format
Full length recombinant KDM1A/LSD1 protein was expressed in Sf9 cells and is supplied in 25mM Hepes pH7.5, 300mM NaCl, 10% Glycerol, 0.04% Triton X-100, and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant LSD1/KDM1A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. MALDI-TOF for LSD1/KDM1A protein activity 3uM H3K4me2 (1-27aa) peptide was incubated with 5nm and 15nm of LSD1/KDM1A protein in reaction buffer containing 50mM Tris-HCl pH 8.0, 50nm FAD for 1 hr at room temperature. Single H3K4me2 peptide was used as negative control. MALDI-TOF was used for detection.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant LSD1 / KDM1A protein gel. LSD1 / KDM1A protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue. MW: 110 kDa Purity: > 91%)
SDS-Page
(Recombinant LSD1 / KDM1A protein gel. LSD1 / KDM1A protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue. MW: 110 kDa Purity: > 91%)
Testing Data
(MALDI-TOF for LSD1 / KDM1A protein activity 3 uM H3K4me2 (1-27aa) peptide was incubated with 5 nM and 15 nM of LSD1 / KDM1A protein in reaction buffer containing 50 mM Tris-HCl pH 8.0, 50 nM FAD for 1 hr at room temperature. Single H3K4me2 peptide was used as negative control. MALDI-TOF was used for detection.)
Testing Data
(MALDI-TOF for LSD1 / KDM1A protein activity 3 uM H3K4me2 (1-27aa) peptide was incubated with 5 nM and 15 nM of LSD1 / KDM1A protein in reaction buffer containing 50 mM Tris-HCl pH 8.0, 50 nM FAD for 1 hr at room temperature. Single H3K4me2 peptide was used as negative control. MALDI-TOF was used for detection.)
Related Product Information for LSD1/KDM1A recombinant protein
Short Description: Recombinant LSD1/KDM1A (accession number NP_055828) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with a molecular weight of 97.1 kDa. LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3 monomethyl Lys4 and Histone H3 dimethyl Lys4, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3 monomethyl Lys9 and Histone H3 dimethyl Lys9, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase. Recombinant LSD1/KDM1A can be used to study enzyme kinetics and perform inhibitor screening.
Background: LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3K4me1 and Histone H3K4me2, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3K9me1 and Histone H3K9me2, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase.
Background: LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3K4me1 and Histone H3K4me2, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3K9me1 and Histone H3K9me2, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase.
Product Categories/Family for LSD1/KDM1A recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of 110kDa
Similar Products
Product Notes
The LSD1/KDM1A (Catalog #AAA388143) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "LSD1/KDM1A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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