SDS-Page
(Recombinant PHF8 protein gel. PHF8 protein was run on a 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 121.1 kDa Purity: > 70%)
PHF8 recombinant protein
Recombinant PHF8 protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant PHF8 protein gel. PHF8 protein was run on a 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 121.1 kDa Purity: > 70%)
SDS-Page
(Recombinant PHF8 protein gel. PHF8 protein was run on a 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 121.1 kDa Purity: > 70%)
Testing Data
(HTRF assay for Recombinant PHF8 protein activity. 1 uM H3K4me3K9me1 peptide was incubated with PHF8 / KDM7B protein in reaction buffer for 1 hour at room temperature. Anti-H3K9me0 antibody was used to detect reaction product.)
Testing Data
(HTRF assay for Recombinant PHF8 protein activity. 1 uM H3K4me3K9me1 peptide was incubated with PHF8 / KDM7B protein in reaction buffer for 1 hour at room temperature. Anti-H3K9me0 antibody was used to detect reaction product.)
Testing Data
(HTRF assay for Recombinant PHF8 protein activity. 1 uM H3K4me3K9me1 peptide was incubated with PHF8 / KDM7B protein in reaction buffer for 1 hour at room temperature. Anti-H3K9me0 antibody was used to detect reaction product.)
Testing Data
(HTRF assay for Recombinant PHF8 protein activity. 1 uM H3K4me3K9me1 peptide was incubated with PHF8 / KDM7B protein in reaction buffer for 1 hour at room temperature. Anti-H3K9me0 antibody was used to detect reaction product.)
Background: PHF8 (PHD finger protein 8), also known as Lysine (K)-specific demethylase 7B (KDM7B) and JmjC domain-containing histone demethylation protein 1D-B (JHDM1DB), is a member of the JmjC-containing (Jumonji-C) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. PHF8 has two N-terminal domains, a PHD finger that binds trimethylated lysine 4 of histone H3 (H3K4me3) and a Jumonji domain that demethylates monomethylated H3 Lys9 (H3K9me1), Histone H3 dimethyl Lys9 (H3K9me2), Histone H3 dimethyl Lys27 (H3K27me2)(which are all modifications associated with transcriptional repression) and also Histone H3 dimethyl Lys36 (H3K36me2). PHF8 depletion in mammalian neuronal cells results in decreased expression of follistatin and increased methylation of H3K9 and H3K27. It has also been suggested to function in gene silencing during brain development.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. The protein has an N-terminal PHD finger and a central Jumonji C domain. This gene is thought to function as a transcription activator. Defects in this gene are a cause of syndromic X-linked Siderius type intellectual disability (MRXSSD) and over-expression of this gene is associated with several forms of cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017]
Uniprot Description
Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.
Research Articles on PHF8
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Product Notes
The PHF8 phf8 (Catalog #AAA388149) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PHF8, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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