JMJD2B/KDM4B recombinant protein

Recombinant JMJD2B/KDM4B protein

Cat. Num. AAA388211

• Gene Names: KDM4B; JMJD2B; TDRD14B
• Purity: The recombinant protein is >90% pure by SDS-PAGE.
• Synonyms: JMJD2B/KDM4B; Recombinant JMJD2B/KDM4B protein; Lysine Demethylase 4B; Jumonji Domain-Containing Protein 2B; JHDM3B; JMJD2B/KDM4B recombinant protein

• Host: Baculovirus
• Purity/Purification: The recombinant protein is >90% pure by SDS-PAGE.
• Form/Format: Full length recombinant JMJD2B/KDM4B protein was expressed in Sf9 cells and is supplied in 25mM Hepes pH7.5, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag, His-Tag
• Notes: Recombinant JMJD2B/KDM4B protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Histone Demethylase Assay Conditions: 3.3uM H3K9me3 (aa 1-21) peptide was incubated with different concentrations of recombinant JMJD2B/KDM4B protein in reaction buffer containing 50mM HEPES pH 7.5, 0.02% Triton X100, 100uM 2OG, 100uM Ascorbate, 50uM (NH4)2Fe(SO4)2·6H2O, 1mM TCEP for 1 hours at room temperature. HTRF and MALDI-TOF was used for detection.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant JMJD2B / KDM4B protein gel. JMJD2B / KDM4B protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue. MW: 126.9 kDa Purity: >70%)

Testing Data

(Recombinant JMJD2B / KDM4B protein HTRF activity assay. 3 ?M H3K9me3 peptide was incubated with JMJD2B / KDM4B in reaction buffer for 1 hours at room temperature. JMJD2B / KDM4B enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K9me2) was measured using H3K9me2-specific antibody.)

Related Product Information for JMJD2B/KDM4B recombinant protein

Short Description: Recombinant JMJD2B/KDM4B (accession number NP_055830.1) was expressed in Sf9 cells and contains an N-terminal 6xHis-tag and DYKDDDDK-Tag with an observed molecular weight of 126.9 kDa. The recombinant protein is >90% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: JMJD2B/KDM4B (JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, JHDM3B) is a histone demethylase that has been shown to demethylate trimethylated lysine 9 (K9me3) and lysine 36 (K36me3) residues of histone H3, converting these trimethylated histone residues to dimethylated or monomethylated form. KDM4B has no activity for monomethylated H3K9 and H3K36. JMJD2B/KDM4B is physically associated with the MLL2 methyltransferase complex where it serves as a co-factor for ERa-mediated transcriptional activation.

Product Categories/Family for JMJD2B/KDM4B recombinant protein

Recombinant Proteins; Histone Demethylase (HDM)

NCBI and Uniprot Product Information

• NCBI GI #: 45504380
• NCBI GeneID: 23030
• NCBI Accession #: NP_055830.1
• NCBI GenBank Nucleotide #: NM_015015.2
• UniProt Accession #: O94953; O75274; Q6P3R5; Q9P1V1; Q9UF40; B9EGN8; D6W631
• Molecular Weight: 121,897 Da
• NCBI Official Full Name: lysine-specific demethylase 4B
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 4B
• NCBI Official Symbol: KDM4B
• NCBI Official Synonym Symbols: JMJD2B; TDRD14B
• NCBI Protein Information: lysine-specific demethylase 4B; tudor domain containing 14B; jumonji domain containing 2B; jumonji domain-containing protein 2B; jmjC domain-containing histone demethylation protein 3B
• UniProt Protein Name: Lysine-specific demethylase 4B
• UniProt Gene Name: KDM4B
• UniProt Synonym Gene Names: JHDM3B; JMJD2B; KIAA0876
• UniProt Entry Name: KDM4B_HUMAN

Uniprot Description

JMJD2B: Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate. Belongs to the JHDM3 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Demethylase; EC 1.14.11.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; focal adhesion; nucleus; cell junction

Molecular Function: dioxygenase activity; zinc ion binding

Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; chromatin modification