SDS-Page
(Recombinant JMJD2C / KDM4C protein gel. JMJD2C / KDM4C protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 126.1 kDa Purity: > 90%)
JMJD2C/KDM4C recombinant protein
Recombinant JMJD2C/KDM4C protein
Purity
The recombinant protein is >95% pure by SDS-PAGE.
Synonyms
JMJD2C/KDM4C; Recombinant JMJD2C/KDM4C protein; Lysine Demethylase 4C; Jumonji Domain-Containing Protein 2C; JHDM3C;; JMJD2C/KDM4C recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >95% pure by SDS-PAGE.
Form/Format
Recombinant JMJD2C/KDM4C protein is supplied at a concentration of 0.4ug/ul in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant JMJD2C/KDM4C is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling Histone Demethylase Assay Conditions: 1uM H3K9me3 peptide was incubated with different concentrations of JMJD2C/KDM4C protein in 10ul reaction system containing 50mM HEPES-NaOH pH 7.5, 1mM TCEP, 50uM 2-OG, 50uM Ascorbate, 25uM (NH4)2Fe(SO4)2·6H2O for 1 hour, then 10ul H3K9me2 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant JMJD2C / KDM4C protein gel. JMJD2C / KDM4C protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 126.1 kDa Purity: > 90%)
SDS-Page
(Recombinant JMJD2C / KDM4C protein gel. JMJD2C / KDM4C protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 126.1 kDa Purity: > 90%)
Testing Data
(JMJD2C / KDM4C activity assay.1 uM H3K9me3 peptide was incubated with different concentrations of JMJD2C / KDM4C protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate, 25 uM (NH4)2Fe(SO4)2·6H2O for 1 hour, then 10 ul H3K9me2 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(JMJD2C / KDM4C activity assay.1 uM H3K9me3 peptide was incubated with different concentrations of JMJD2C / KDM4C protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate, 25 uM (NH4)2Fe(SO4)2·6H2O for 1 hour, then 10 ul H3K9me2 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Related Product Information for JMJD2C/KDM4C recombinant protein
Short Description: Recombinant JMJD2C/KDM4C (accession number NP_055876.2) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with an observed molecular weight of 124.1 kDa. KDM4C (lysine (K)-specific demethylase 4C), also known as JMJD2C (Jumonji Domain Containing 2C) is a nuclear protein that functions as a trimethylation-specific histone demethylase that preferentially demethylates trimethylated lysine 9 (K9me3) and lysine 36 (K36me3) residues of histone H3, converting these trimethylated histone residues to dimethylated form. KDM4C has no activity for mono- and dimethylated H3K9 and H3K36. Chromosomal aberrations and overexpression of KDM4C are associated with esophageal squamous cell carcinoma. KDM4C regulates colonosphere formation in colorectal cancers by mediating the crosstalk between Wnt and Notch signaling pathways. Recombinant JMJD2C/KDM4C can be used for enzyme kinetics and inhibitor screening studies.
Background: KDM4C (lysine (K)-specific demethylase 4C), also known as JMJD2C (Jumonji Domain Containing 2C) is a nuclear protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 9 (K9me3) and lysine 36 (K36me3) residues of histone H3, converting these trimethylated histone residues to mono- or dimethylated form. JMJD2C/KDM4C has no activity for monomethylated H3K9 and H3K36. Chromosomal aberrations and overexpression of KDM4C are associated with esophageal squamous cell carcinoma. KDM4C regulates colonosphere formation in colorectal cancers by mediating the crosstalk between Wnt and Notch signaling pathways.
Background: KDM4C (lysine (K)-specific demethylase 4C), also known as JMJD2C (Jumonji Domain Containing 2C) is a nuclear protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 9 (K9me3) and lysine 36 (K36me3) residues of histone H3, converting these trimethylated histone residues to mono- or dimethylated form. JMJD2C/KDM4C has no activity for monomethylated H3K9 and H3K36. Chromosomal aberrations and overexpression of KDM4C are associated with esophageal squamous cell carcinoma. KDM4C regulates colonosphere formation in colorectal cancers by mediating the crosstalk between Wnt and Notch signaling pathways.
Product Categories/Family for JMJD2C/KDM4C recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of 124.1kDa
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Product Notes
The JMJD2C/KDM4C (Catalog #AAA388172) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "JMJD2C/KDM4C, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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