SDS-Page
(Recombinant FBXL10 / KDM2B protein gel. FBXL10 / KDM2B protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.)
FBXL10/KDM2B recombinant protein
Recombinant FBXL10/KDM2B protein
Gene Names
KDM2B; CXXC2; Fbl10; PCCX2; FBXL10; JHDM1B
Purity
The recombinant protein is >75% pure by SDS-PAGE.
Synonyms
FBXL10/KDM2B; Recombinant FBXL10/KDM2B protein; CXXC2; Fbl10; PCCX2; JHDM1B; FBXL10/KDM2B recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >75% pure by SDS-PAGE.
Form/Format
Full length recombinant FBXL10/KDM2B protein expressed in Sf9 cells and is supplied in 25mM HEPES-NaOH, pH7.5, 300mM NaCl, 5% glycerol and 0.2mg/ml 3x DYKDDDDK peptide.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant FBXL10/KDM2B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Histone Demethylase Assay Conditions: 50mM HEPES pH 7.5, 0.02% Triton X100, 100uM 2OG, 100uM Ascorbate, 50uM (NH4)2Fe(SO4)2·6H2O, 1mM TCEP, 300nm Recombinant FBXL10/KDM2B protein, and 3.3uM H3K36me2 peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant FBXL10 / KDM2B protein gel. FBXL10 / KDM2B protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.)
SDS-Page
(Recombinant FBXL10 / KDM2B protein gel. FBXL10 / KDM2B protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.)
Related Product Information for FBXL10/KDM2B recombinant protein
Short Description: Recombinant FBXL10/KDM2B (accession number NP_115979.3) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with an observed molecular weight of 155.9 kDa. KDM2B (lysine (K)-specific demethylase 2B), also known as FBXL10 (F-box and leucine-rich repeat protein 10) is a histone demethylase that preferentially demethylates trimethylated lysine 4 (K4me3) and dimethylated lysine 36 (K36me2) of histone H3. KDM2B displays weak or no activity for mono- and trimethylated H3K36. KDM2B preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which results in inhibition of cell growth and proliferation. KDM2B may also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Recombinant FBXL10/KDM2B can be used for enzyme kinetics and inhibitor screening studies.
Background: KDM2B (lysine (K)-specific demethylase 2B), also known as FBXL10 (F-box and leucine-rich repeat protein 10) is a histone demethylase that preferentially demethylates trimethylated lysine 4 (K4me3) and dimethylated lysine 36 (K36me2) of histone H3. KDM2B displays weak or no activity for mono- and trimethylated H3K36. KDM2B preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which results in inhibition of cell growth and proliferation. KDM2B may also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Background: KDM2B (lysine (K)-specific demethylase 2B), also known as FBXL10 (F-box and leucine-rich repeat protein 10) is a histone demethylase that preferentially demethylates trimethylated lysine 4 (K4me3) and dimethylated lysine 36 (K36me2) of histone H3. KDM2B displays weak or no activity for mono- and trimethylated H3K36. KDM2B preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which results in inhibition of cell growth and proliferation. KDM2B may also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Product Categories/Family for FBXL10/KDM2B recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
lysine-specific demethylase 2B isoform a
NCBI Official Synonym Full Names
lysine demethylase 2B
NCBI Official Symbol
KDM2B
NCBI Official Synonym Symbols
CXXC2; Fbl10; PCCX2; FBXL10; JHDM1B
NCBI Protein Information
lysine-specific demethylase 2B
NCBI Description
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
Research Articles on FBXL10/KDM2B
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Product Notes
The FBXL10/KDM2B (Catalog #AAA388169) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "FBXL10/KDM2B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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