SDS-Page
(Recombinant KDM7A protein gel. KDM7A protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.)
KDM7A recombinant protein
Recombinant KDM7A protein
Gene Names
KDM7A; JHDM1D
Purity
The recombinant protein is >70% pure by SDS-PAGE.
Synonyms
KDM7A; Recombinant KDM7A protein; Lysine Demethylase 7A; Jumonji C Domain Containing Histone Demethylase; JHDM1D; KDM7A recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >70% pure by SDS-PAGE.
Form/Format
Recombinant KDM7A protein is supplied in 25mM HEPES pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant KDM7A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Protein Function: H3K9me2, H3K27me2 and H4K20me1 demethylase.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant KDM7A protein gel. KDM7A protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-Page
(Recombinant KDM7A protein gel. KDM7A protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.)
Testing Data
(HTRF assay for KDM7A activity. 1 uM H3K9me2 peptide was incubated with KDM7A protein in reaction buffer with or without 0.02% Triton X-100, respectively, for 1 hour at room temperature. KDM7A enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K9me0) was measured using H3K9me0-specific antibody. The results show that 0.02% Triton X-100 slightly inhibits the activity of KDM7A.)
Testing Data
(HTRF assay for KDM7A activity. 1 uM H3K9me2 peptide was incubated with KDM7A protein in reaction buffer with or without 0.02% Triton X-100, respectively, for 1 hour at room temperature. KDM7A enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K9me0) was measured using H3K9me0-specific antibody. The results show that 0.02% Triton X-100 slightly inhibits the activity of KDM7A.)
Testing Data
(HTRF assay for KDM7A activity. 1 uM H3K9me2 peptide was incubated with KDM7A protein in reaction buffer with or without 0.02% Triton X-100, respectively, for 1 hour at room temperature. KDM7A enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K9me0) was measured using H3K9me0-specific antibody. The results show that 0.02% Triton X-100 slightly inhibits the activity of KDM7A.)
Testing Data
(HTRF assay for KDM7A activity. 1 uM H3K9me2 peptide was incubated with KDM7A protein in reaction buffer with or without 0.02% Triton X-100, respectively, for 1 hour at room temperature. KDM7A enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K9me0) was measured using H3K9me0-specific antibody. The results show that 0.02% Triton X-100 slightly inhibits the activity of KDM7A.)
Related Product Information for KDM7A recombinant protein
Short Description: Recombinant KDM7A (accession number NP_085150.1) was expressed in Sf9 and contains an N-terminal DYKDDDDK-Tag with a molecular weight of 109.6 kDa. The recombinant protein is >70% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: KDM7A (Lysine (K)-specific demethylase 7A), also known as Jumonji C Domain Containing Histone Demethylase 1 Homolog D (JHDM1D), is a member of the JmjC-containing (Jumonji-C) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. KDM7A has two N-terminal domains, a PHD finger that binds tri-methylated lysine 4 of histone H3 (H3K4me3) and a Jumonji domain that demethylates Histone H3 dimethyl Lys9 (H3K9me2) and Histone H3 dimethyl Lys27 (H3K27me2)(which are all modifications associated with transcriptional repression). The PHD domain of KDM7A specifically binds H3K4me3, affecting histone demethylase specificity. In the presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. It also demethylates H3K9me2 in absence of H3K4me3. KDM7A demethylates H4K20me1 only when nucleosome is used as a substrate.
Background: KDM7A (Lysine (K)-specific demethylase 7A), also known as Jumonji C Domain Containing Histone Demethylase 1 Homolog D (JHDM1D), is a member of the JmjC-containing (Jumonji-C) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. KDM7A has two N-terminal domains, a PHD finger that binds tri-methylated lysine 4 of histone H3 (H3K4me3) and a Jumonji domain that demethylates Histone H3 dimethyl Lys9 (H3K9me2) and Histone H3 dimethyl Lys27 (H3K27me2)(which are all modifications associated with transcriptional repression). The PHD domain of KDM7A specifically binds H3K4me3, affecting histone demethylase specificity. In the presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. It also demethylates H3K9me2 in absence of H3K4me3. KDM7A demethylates H4K20me1 only when nucleosome is used as a substrate.
Product Categories/Family for KDM7A recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,001 Da
NCBI Official Full Name
Homo sapiens lysine (K)-specific demethylase 7A (KDM7A), mRNA
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 7A
NCBI Official Symbol
KDM7A
NCBI Official Synonym Symbols
JHDM1D
NCBI Protein Information
lysine-specific demethylase 7A; histone lysine demethylase JHDM1D; jmjC domain-containing histone demethylation protein 1D; jumonji C domain containing histone demethylase 1 homolog D; jumonji C domain-containing histone demethylase 1 homolog D
Protein Family
Research Articles on KDM7A
Similar Products
Product Notes
The KDM7A (Catalog #AAA388278) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KDM7A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
