Testing Data
General Transketolase Assay Kit
Transketolase Activity Assay Kit (Fluorometric)
Reactivity
General
Synonyms
Transketolase; Transketolase Activity Assay Kit (Fluorometric); Transketolase Assay; Transketolase Kit (Fluorometric); Transketolase Activity Assay Kit; Transketolase Activity; Transketolase Activity Assay (Fluorometric); Transketolase Activity Kit; TKT; Transketolase Kit; Transketolase Activity Kit (Fluorometric); Transketolase Activity Assay; Transketolase Activity (Fluorometric); Transketolase assay kit
Reactivity
General
Samples
Tissue lysate (e.g. Liver tissue), Cell lysate, Recombinant enzyme, Purified protein
Detection Method
Fluorescence: Ex/Em 535/587nm
Features & Benefits
Plate-based fluorometric assay
Simple, The assay can detect as low as 5 uU of TKT activity in biological samples
Measure Transketolase activity
For measuring TKT activity in biological samples
Simple, The assay can detect as low as 5 uU of TKT activity in biological samples
Measure Transketolase activity
For measuring TKT activity in biological samples
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Transketolase assay kit
Transketolase (EC 2.2.1.1) is an important enzyme of the non-oxidative branch of the pentose phosphate pathway, which metabolizes glucose to form pentose and NADPH. It is also involved in the photosynthetic Calvin cycle in plants and autotrophic bacteria. Transketolase (TKT) catalyzes two reactions in the pentose phosphate pathway, both of which are involved in the transfer of a 2-carbon glycoaldehyde fragment from a alpha-keto pentose sugar such as xylulose-5-phosphate to another aldose sugar such as ribose-5-phosphate or erythrose-4-phosphate. It is present in the cytosol of most tissues and its activity depends on the binding of thiamin pyrophosphate, a derivative of thiamin (Vitamin B1). Therefore TKT activity is decreased in thiamine deficiency and may be used in the test of Wernicke-Korsakoff syndrome. Transketolase Activity Assay Kit is a simple, plate-based fluorometric assay for measuring TKT activity in biological samples. In this assay, TKT transfers a two-carbon group from a donor keto sugar to an acceptor aldose sugar. The product formed converts a non-fluorescent probe to a fluorescent product via an enzymatic reaction in the presence of a developer and an enzyme mix. The assay can detect as low as 5 uU of TKT activity in biological samples.
Product Categories/Family for Transketolase assay kit
Similar Products
Product Notes
The General Transketolase (Catalog #AAA849580) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849580 Assay Kit recognizes General Transketolase. It is sometimes possible for the material contained within the vial of "Transketolase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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